Abstract

It remains unclear why the ST258 lineage is the most prevalent cause of multidrug resistant K. pneumoniae infections in the United States and other countries. To that end, we continued our research directed to better understand of the basis for the success of ST258. These studies included further analysis of region of recombination that encompasses the genes encoding capsule polysaccharide biosynthesis machinery. In addition, we studied the interaction of carbapenem-resistant Klebsiella pneumoniae (ST258) with human neutrophils to test the hypothesis that this strain has enhanced capacity to circumvent killing by the innate immune system. Our findings will accelerate research on novel diagnostic, therapeutic, and vaccine strategies designed to prevent and/or treat infections caused by multidrug resistant K. pneumoniae.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIAAI001195-04
Application #
9566761
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
2017
Total Cost
Indirect Cost

  Institution

Name
Niaid Extramural Activities
Department
Type
DUNS #
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State
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  Publications

Kobayashi, Scott D; Porter, Adeline R; Freedman, Brett et al. (2018) Antibody-Mediated Killing of Carbapenem-Resistant ST258 Klebsiella pneumoniae by Human Neutrophils. MBio 9:
DeLeo, Frank R; Kobayashi, Scott D; Porter, Adeline R et al. (2017) Survival of Carbapenem-Resistant Klebsiella pneumoniae Sequence Type 258 in Human Blood. Antimicrob Agents Chemother 61:
Kobayashi, Scott D; Porter, Adeline R; Dorward, David W et al. (2016) Phagocytosis and Killing of Carbapenem-Resistant ST258 Klebsiella pneumoniae by Human Neutrophils. J Infect Dis 213:1615-22
Brinkworth, Amanda J; Hammer, Carl H; Olano, L Renee et al. (2015) Identification of Outer Membrane and Exoproteins of Carbapenem-Resistant Multilocus Sequence Type 258 Klebsiella pneumoniae. PLoS One 10:e0123219
Chen, Liang; Chavda, Kalyan D; DeLeo, Frank R et al. (2015) Genome Sequence of a Klebsiella pneumoniae Sequence Type 258 Isolate with Prophage-Encoded K. pneumoniae Carbapenemase. Genome Announc 3:
Chen, Liang; Mathema, Barun; Chavda, Kalyan D et al. (2014) Carbapenemase-producing Klebsiella pneumoniae: molecular and genetic decoding. Trends Microbiol 22:686-96
Chen, Liang; Chavda, Kalyan D; Findlay, Jacqueline et al. (2014) Multiplex PCR for identification of two capsular types in epidemic KPC-producing Klebsiella pneumoniae sequence type 258 strains. Antimicrob Agents Chemother 58:4196-9
Chen, Liang; Mathema, Barun; Pitout, Johann D D et al. (2014) Epidemic Klebsiella pneumoniae ST258 is a hybrid strain. MBio 5:e01355-14
Deleo, Frank R; Chen, Liang; Porcella, Stephen F et al. (2014) Molecular dissection of the evolution of carbapenem-resistant multilocus sequence type 258 Klebsiella pneumoniae. Proc Natl Acad Sci U S A 111:4988-93