We continue to investigate and publish our findings on the microbiota in patients with atopic dermatitis in contrast to healthy individuals. We study bacterial, fungal, and viral components of the skin microbiome and have expanded our sequencing repertoire beyond bacterial amplicon sequencing to include fungal amplicon sequencing and shotgun metagenomic sequencing. We continue to study the skin microbiome in patients with atopic dermatitis and with primary immunodeficiencies associated with dermatitis (hyper-IgE syndrome and Wiskott-Aldrich syndrome), in addition to follow-up sampling visits from previously enrolled subjects. The sequencing for this project initially was funded by the National Institutes of Health Roadmap Project, The Human Microbiome Project. In the last fiscal year, we have expanded our work to study these patient populations using more advanced sequencing techniques.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIABC010938-09
Application #
9343744
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
9
Fiscal Year
2016
Total Cost
Indirect Cost
Name
Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code
Kennedy, Elizabeth A; Connolly, Jennifer; Hourihane, Jonathan O'B et al. (2017) Skin microbiome before development of atopic dermatitis: Early colonization with commensal staphylococci at 2 months is associated with a lower risk of atopic dermatitis at 1 year. J Allergy Clin Immunol 139:166-172
Kong, Heidi H; Andersson, Björn; Clavel, Thomas et al. (2017) Performing Skin Microbiome Research: A Method to the Madness. J Invest Dermatol 137:561-568
Kong, Heidi H; Morris, Alison (2017) The emerging importance and challenges of the human mycobiome. Virulence 8:310-312
Byrd, Allyson L; Deming, Clay; Cassidy, Sara K B et al. (2017) Staphylococcus aureus and Staphylococcus epidermidis strain diversity underlying pediatric atopic dermatitis. Sci Transl Med 9:
Shah, Nirali N; Freeman, Alexandra F; Su, Helen et al. (2017) Haploidentical Related Donor Hematopoietic Stem Cell Transplantation for Dedicator-of-Cytokinesis 8 Deficiency Using Post-Transplantation Cyclophosphamide. Biol Blood Marrow Transplant 23:980-990
Tsai, Yu-Chih; Conlan, Sean; Deming, Clayton et al. (2016) Resolving the Complexity of Human Skin Metagenomes Using Single-Molecule Sequencing. MBio 7:e01948-15
Bosch, Thomas C G; Zengler, Karsten; Naik, Shruti et al. (2016) FRT - FONDATION RENE TOURAINE: An International Foundation For Dermatology. Exp Dermatol 25:917-932
Kong, Heidi H (2016) Details Matter: Designing Skin Microbiome Studies. J Invest Dermatol 136:900-902
Oh, Julia; Byrd, Allyson L; Park, Morgan et al. (2016) Temporal Stability of the Human Skin Microbiome. Cell 165:854-66
Jo, Jay-Hyun; Kennedy, Elizabeth A; Kong, Heidi H (2016) Research Techniques Made Simple: Bacterial 16S Ribosomal RNA Gene Sequencing in Cutaneous Research. J Invest Dermatol 136:e23-e27

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