We are purifying populations of putative cancer stem cells from hepatocellular carcinoma, cholangiocarcinoma, pancreatic adenocarcinoma, melanoma and gastric cancer using a novel functional approach. We preliminarrily were able to generate a polyclonal antibody spesific for cancer stem cells. Now we are in the proccess of generating a monoclonal antibody. Subsequently, we will purify the specific protein and will test the antibody against a variety of various cancers. We hope to study the predictive value of this antibody as an early predictor.
|Ray, Satyajit; Langan, Russell C; Mullinax, John E et al. (2012) Establishment of human ultra-low passage colorectal cancer cell lines using spheroids from fresh surgical specimens suitable for in vitro and in vivo studies. J Cancer 3:196-206|
|Mullinax, John E; Allins, Alexander; Avital, Itzhak (2011) Laparoscopic appendectomy for Amyand's hernia: a modern approach to a historic diagnosis. J Gastrointest Surg 15:533-5|
|Avital, Itzhak (2011) Regional chemotherapy for pancreatic cancer. J Surg Oncol 104:453|