In this project we investigate wide array of cancers: hepatocellular carcinoma, cholangiocarcinoma, pancreatic adenocarcinoma, breast cancer, melanoma, and gastric cancers This project has two prongs: One, where we use a functional approach to identify the """"""""best"""""""" cancer stem cell usiong all previously described methodes and markers and subjecting them to a rigorous tesing methodology. Once identified we will try to target these cells via the specific marker in attempt to generate novel cancer therapy. Two, using a novel methodology we try to isolate the self renwing cells via asymmetric division in various cancers and determine whether these are the """"""""fitest"""""""" cancer stem cells

National Institute of Health (NIH)
National Cancer Institute (NCI)
Investigator-Initiated Intramural Research Projects (ZIA)
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National Cancer Institute Division of Basic Sciences
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Xin, Hong-Wu; Ambe, Chenwi M; Miller, Tyler C et al. (2016) Liver Label Retaining Cancer Cells Are Relatively Resistant to the Reported Anti-Cancer Stem Cell Drug Metformin. J Cancer 7:1142-51
Xin, Hong-Wu; Ambe, Chenwi M; Ray, Satyajit et al. (2013) Wnt and the cancer niche: paracrine interactions with gastrointestinal cancer cells undergoing asymmetric cell division. J Cancer 4:447-57
Ray, Satyajit; Langan, Russell C; Mullinax, John E et al. (2012) Establishment of human ultra-low passage colorectal cancer cell lines using spheroids from fresh surgical specimens suitable for in vitro and in vivo studies. J Cancer 3:196-206
Mullinax, John E; Allins, Alexander; Avital, Itzhak (2011) Laparoscopic appendectomy for Amyand's hernia: a modern approach to a historic diagnosis. J Gastrointest Surg 15:533-5
Avital, Itzhak (2011) Regional chemotherapy for pancreatic cancer. J Surg Oncol 104:453