Abstract

We collaborated with Larry Tabak's lab to study developmental functions of protein O-glycosylation in S. purpuratus embryos. We identified 13 SpGalNAc-T genes and analyses their expression patterns during development using whole-mount in situ hybridization. Developmental function of selected GalNac-Ts was analyzed by morpholine oligonucleotide-mediated knockdown of expression. One phenotype has been characterized in morphants in which SpGalNAc-T13 expression was attenuated. This results in a deficiency of embryonic skeleton and neurons, suggesting that mucin-type O-glycans play essential roles during embryonic development in S. purpuratus.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIADE000712-13
Application #
9566787
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
13
Fiscal Year
2017
Total Cost
Indirect Cost

  Institution

Name
Dental & Craniofacial Research
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Famiglietti, Amber L; Wei, Zheng; Beres, Thomas M et al. (2017) Characterization and expression analysis of Galnts in developing Strongylocentrotus purpuratus embryos. PLoS One 12:e0176479
Wei, Zheng; Angerer, Lynne M; Angerer, Robert C (2016) Neurogenic gene regulatory pathways in the sea urchin embryo. Development 143:298-305
Sethi, Aditya J; Angerer, Robert C; Angerer, Lynne M (2014) Multicolor labeling in developmental gene regulatory network analysis. Methods Mol Biol 1128:249-62
Range, Ryan C; Angerer, Robert C; Angerer, Lynne M (2013) Integration of canonical and noncanonical Wnt signaling pathways patterns the neuroectoderm along the anterior-posterior axis of sea urchin embryos. PLoS Biol 11:e1001467
Sethi, Aditya J; Wikramanayake, Radhika M; Angerer, Robert C et al. (2012) Sequential signaling crosstalk regulates endomesoderm segregation in sea urchin embryos. Science 335:590-3
Yaguchi, Junko; Angerer, Lynne M; Inaba, Kazuo et al. (2012) Zinc finger homeobox is required for the differentiation of serotonergic neurons in the sea urchin embryo. Dev Biol 363:74-83
Wei, Zheng; Range, Ryan; Angerer, Robert et al. (2012) Axial patterning interactions in the sea urchin embryo: suppression of nodal by Wnt1 signaling. Development 139:1662-9
Wei, Zheng; Angerer, Robert C; Angerer, Lynne M (2011) Direct development of neurons within foregut endoderm of sea urchin embryos. Proc Natl Acad Sci U S A 108:9143-7
Adams, Diane K; Sewell, Mary A; Angerer, Robert C et al. (2011) Rapid adaptation to food availability by a dopamine-mediated morphogenetic response. Nat Commun 2:592
Yaguchi, Shunsuke; Yaguchi, Junko; Wei, Zheng et al. (2011) Fez function is required to maintain the size of the animal plate in the sea urchin embryo. Development 138:4233-43

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