Abstract

Very little is known about heme transport in eukaryotes and a heme-specific transport uptake systems have not been identified in yeast. Heme is synthesized in mitochondria, yet the cofactor is used in compartments throughout the cell. Heme is highly hydrophobic and is unlikely to be released into the aqueous environment of the cytosol, suggesting that mechanisms exist for the intracellular trafficking of heme. We are currently characterizing the heme efflux activity of the putative mitochondrial heme exporter of humans, FLVCR1b.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIADK054513-08
Application #
8939623
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
8
Fiscal Year
2014
Total Cost
Indirect Cost

  Institution

Name
U.S. National Inst Diabetes/Digst/Kidney
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Rostovtseva, Tatiana K; Gurnev, Philip A; Protchenko, Olga et al. (2015) ?-Synuclein Shows High Affinity Interaction with Voltage-dependent Anion Channel, Suggesting Mechanisms of Mitochondrial Regulation and Toxicity in Parkinson Disease. J Biol Chem 290:18467-77
Cyert, Martha S; Philpott, Caroline C (2013) Regulation of cation balance in Saccharomyces cerevisiae. Genetics 193:677-713
Huynh, Chau; Yuan, Xiaojing; Miguel, Danilo C et al. (2012) Heme uptake by Leishmania amazonensis is mediated by the transmembrane protein LHR1. PLoS Pathog 8:e1002795
Philpott, Caroline C; Leidgens, Sébastien; Frey, Avery G (2012) Metabolic remodeling in iron-deficient fungi. Biochim Biophys Acta 1823:1509-20
Yuan, Xiaojing; Protchenko, Olga; Philpott, Caroline C et al. (2012) Topologically conserved residues direct heme transport in HRG-1-related proteins. J Biol Chem 287:4914-24
Boretsky, Yuriy R; Pynyaha, Yuriy V; Boretsky, Volodymyr Y et al. (2011) Identification of the genes affecting the regulation of riboflavin synthesis in the flavinogenic yeast Pichia guilliermondii using insertion mutagenesis. FEMS Yeast Res 11:307-14