Abstract

This project describes the role of the lab of Stephanie London in the Laboratory of Respiratory Biology in support of her epidemiologic studies. The laboratory is engaged in genetic and epigenetic analyses to support Dr. Londons epidemiologic projects. The lab supports follow-up of findings from our genome wide association studies of childhood asthma as well as adult pulmonary function. Human genome wide association studies are well designed to identify novel association with pulmonary and other traits. GWAS studies take advantage of correlation across the genome to find these associations. But this correlation makes it difficult to identify the causal variants or even the causal genes. Experimental models can help establish that a GWAS gene is causal. We had previously published two papers where we confirmed human GWAS findings for HTR4 in a knock out mouse model (PMID: 25342126 and PMID: 28130264). We developed conditional mouse models for LRP1. A paper will be submitted soon. We also developed a model for ADAM19 and work is underway to characterize this mouse model.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Investigator-Initiated Intramural Research Projects (ZIA)
Project #
1ZIAES025045-20
Application #
10008715
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
20
Fiscal Year
2019
Total Cost
Indirect Cost

  Institution

Name
National Institute of Environmental Health Sciences
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Nichols, Cody E; Shepherd, Danielle L; Hathaway, Quincy A et al. (2018) Reactive oxygen species damage drives cardiac and mitochondrial dysfunction following acute nano-titanium dioxide inhalation exposure. Nanotoxicology 12:32-48
House, John S; Nichols, Cody E; Li, Huiling et al. (2017) Vagal innervation is required for pulmonary function phenotype in Htr4(-/-) mice. Am J Physiol Lung Cell Mol Physiol 312:L520-L530
Sharma, Sunita; Kho, Alvin T; Chhabra, Divya et al. (2015) Glucocorticoid genes and the developmental origins of asthma susceptibility and treatment response. Am J Respir Cell Mol Biol 52:543-53
Obeidat, Ma'en; Hao, Ke; Bossé, Yohan et al. (2015) Molecular mechanisms underlying variations in lung function: a systems genetics analysis. Lancet Respir Med 3:782-95
Yan, Xiting; Chu, Jen-Hwa; Gomez, Jose et al. (2015) Noninvasive analysis of the sputum transcriptome discriminates clinical phenotypes of asthma. Am J Respir Crit Care Med 191:1116-25
Magnus, Maria C; Håberg, Siri E; Karlstad, Øystein et al. (2015) Grandmother's smoking when pregnant with the mother and asthma in the grandchild: the Norwegian Mother and Child Cohort Study. Thorax 70:237-43
Gharib, Sina A; Loth, Daan W; Soler Artigas, María et al. (2015) Integrative pathway genomics of lung function and airflow obstruction. Hum Mol Genet 24:6836-48
House, John S; Li, Huiling; DeGraff, Laura M et al. (2015) Genetic variation in HTR4 and lung function: GWAS follow-up in mouse. FASEB J 29:323-35
Reddy, Poovendhree; Naidoo, Rajen N; Robins, Thomas G et al. (2012) GSTM1 and GSTP1 gene variants and the effect of air pollutants on lung function measures in South African children. Am J Ind Med 55:1078-86
Wilk, Jemma B; Shrine, Nick R G; Loehr, Laura R et al. (2012) Genome-wide association studies identify CHRNA5/3 and HTR4 in the development of airflow obstruction. Am J Respir Crit Care Med 186:622-32

Showing the most recent 10 out of 21 publications