Extension of the endoplasmic reticulum (ER) into dendritic spines of Purkinje neurons (PNs) is required for cerebellar synaptic plasticity and is disrupted in animals with null mutations in Myo5a, the gene encoding myosin-Va. Notably, the mechanism ensuring the ERs localization to spines has not been unraveled. While it has been proposed that animal class V myosins localize organelles by tethering them to the actin cytoskeleton, we demonstrate here that myosin-Va acts as a point-to-point organelle transporter to pull ER as cargo into PN spines. We show that the myosin accumulates at the ER tip as the organelle moves into spines, and that the myosins ability to hydrolyze ATP is required for the ERs movement into spines. Importantly, attenuation of the myosins ability to move along actin filaments reduces the maximum velocity of ER movement, providing direct evidence that myosin-Va drives ER motility. Thus, we establish for the first time within animal cells that an actin-based motor moves ER, and we uncover the mechanism that mediates ER localization to PN spines, a prerequisite for synaptic plasticity. Capping Protein (CP) is a ubiquitously-expressed, 62 kDa heterodimer that binds the barbed end of the actin filament with very high affinity (0.1nM) to prevent further monomer addition and loss. CARMIL is a multi-domain protein, present from protozoa to mammals, that binds CP and appears to be important for normal actin dynamics in vivo. CARMILs CP binding site resides in its CAH3 domain, a small domain that resides at or near the proteins C-terminus. CAH3 binds CP with 1 nM affinity, resulting in a complex with weak barbed end capping activity (30-200 nM). Solution assays and single-molecule imaging show that CAH3 can bind to CP already present on the barbed end, causing a 300X increase in CPs rate of dissociation from the end i.e. uncapping. Here we used nuclear magnetic resonance (NMR) and intermolecular paramagnetic relaxation enhancement experiments to define the molecular interaction between the minimal CAH3 domain (CAH3a/b) of mouse CARMIL-1 (mCARMIL-1) and CP. Specifically, we show that the CAH3a sub-domain is required for CAH3s high affinity interaction with CP. This interaction consists largely of electrostatic interactions between the highly basic CAH3a sub-domain and a complementary acidic groove on CP that lies opposite its actin-binding surface. This CAH3a: CP interaction serves to orient the CAH3b sub-domain, which we show to also be required for potent anti-CP activity, directly adjacent to the basic patch on CP, shown previously to be required for CPs association to and high affinity interaction with the barbed end. The importance of specific residue interactions between CP and CAH3a/b were confirmed by site-directed mutagenesis of both proteins. Together, these results offer a mechanistic explanation for the barbed end uncapping activity of CARMIL and they identify the basic patch on CP as a crucial regulatory site. Capping protein (CP) is a ubiquitously expressed, heterodimeric, 62 kDa protein that binds the barbed end of the actin filament with high affinity to block further filament elongation. Myotrophin/V-1 is a 13 kDa, ankyrin repeat-containing protein that binds CP tightly, sequestering it in a totally inactive complex in vitro. Here we elucidate the molecular interaction between CP and V-1 by nuclear magnetic resonance (NMR). Specifically, chemical shift mapping and intermolecular paramagnetic relaxation enhancement experiments reveal that the ankyrin loops of V-1, which are essential for V-1:CP interaction, bind the basic patch near the joint of CPs alpha-tentacle, that was shown previously to drive most of CPs association with and affinity for the barbed end. Consistently, site-directed mutagenesis of CP shows that the strong electrostatic binding site for CP on the barbed end and V-1 compete for this basic patch on CP. These results can explain how V-1 inactivates barbed end capping by CP and why V-1 is incapable of uncapping CP-capped actin filaments, the two signature biochemical activities of V-1.

Project Start
Project End
Budget Start
Budget End
Support Year
27
Fiscal Year
2010
Total Cost
$2,184,928
Indirect Cost
Name
National Heart, Lung, and Blood Institute
Department
Type
DUNS #
City
State
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Hammer, John A (2018) Myosin goes for blood. Proc Natl Acad Sci U S A 115:4813-4815
Alexander, Christopher J; Wagner, Wolfgang; Copeland, Neal G et al. (2018) Creation of a myosin Va-TAP tagged mouse and identification of potential myosin Va-interacting proteins in the cerebellum. Cytoskeleton (Hoboken) :
Heimsath Jr, Ernest G; Yim, Yang-In; Mustapha, Mirna et al. (2017) Myosin-X knockout is semi-lethal and demonstrates that myosin-X functions in neural tube closure, pigmentation, hyaloid vasculature regression, and filopodia formation. Sci Rep 7:17354
Bruun, Kyle; Beach, Jordan R; Heissler, Sarah M et al. (2017) Re-evaluating the roles of myosin 18A? and F-actin in determining Golgi morphology. Cytoskeleton (Hoboken) 74:205-218
Burman, Jonathon L; Pickles, Sarah; Wang, Chunxin et al. (2017) Mitochondrial fission facilitates the selective mitophagy of protein aggregates. J Cell Biol 216:3231-3247
Varadarajan, Ramya; Hammer, John A; Rusan, Nasser M (2017) A centrosomal scaffold shows some self-control. J Biol Chem 292:20410-20411
Beach, Jordan R; Bruun, Kyle S; Shao, Lin et al. (2017) Actin dynamics and competition for myosin monomer govern the sequential amplification of myosin filaments. Nat Cell Biol 19:85-93
Beach, Jordan R; Hammer 3rd, John A (2015) Myosin II isoform co-assembly and differential regulation in mammalian systems. Exp Cell Res 334:2-9
Li, Dong; Shao, Lin; Chen, Bi-Chang et al. (2015) ADVANCED IMAGING. Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics. Science 349:aab3500
Billington, Neil; Beach, Jordan R; Heissler, Sarah M et al. (2015) Myosin 18A coassembles with nonmuscle myosin 2 to form mixed bipolar filaments. Curr Biol 25:942-8

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