We continue to examine how Arf6 functions at the plasma membrane to regulate cell surface structure and membrane traffic. GTP-binding proteins like Arf6 cycle between GTP-bound, active and GDP-bound, inactive forms. Sutdies examining roles for Arf GEFs and GAPs are ongoing. Activation of Arfs is catalyzed by guanine nucleotide exchange factors (GEFs) and inactivation catalyzed by GTPase activating proteins (GAPs). Our surprising finding that Arf6-GTP recruits a family of Arf GEFs to the cell surface and leads to the increased activation of Arf1 there has led us to examine in more detail possible roles for Golgi-associated Arfs at the cell surface.

Project Start
Project End
Budget Start
Budget End
Support Year
16
Fiscal Year
2011
Total Cost
$214,100
Indirect Cost
Name
National Heart, Lung, and Blood Institute
Department
Type
DUNS #
City
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Donaldson, Julie G; Johnson, Debra L; Dutta, Dipannita (2016) Rab and Arf G Proteins in Endosomal Trafficking & Cell Surface Homeostasis. Small GTPases :0
Dutta, Dipannita; Donaldson, Julie G (2015) Rab and Arf G proteins in endosomal trafficking. Methods Cell Biol 130:127-38
Caviston, Juliane P; Cohen, Lee Ann; Donaldson, Julie G (2014) Arf1 and Arf6 promote ventral actin structures formed by acute activation of protein kinase C and Src. Cytoskeleton (Hoboken) 71:380-94
Sakurai, Atsuko; Jian, Xiaoying; Lee, Charity J et al. (2011) Phosphatidylinositol-4-phosphate 5-kinase and GEP100/Brag2 protein mediate antiangiogenic signaling by semaphorin 3E-plexin-D1 through Arf6 protein. J Biol Chem 286:34335-45
Donaldson, Julie G; Jackson, Catherine L (2011) ARF family G proteins and their regulators: roles in membrane transport, development and disease. Nat Rev Mol Cell Biol 12:362-75
Cohen, Lee Ann; Donaldson, Julie G (2010) Analysis of Arf GTP-binding protein function in cells. Curr Protoc Cell Biol Chapter 3:Unit 14.12.1-17
Grant, Barth D; Donaldson, Julie G (2009) Pathways and mechanisms of endocytic recycling. Nat Rev Mol Cell Biol 10:597-608
Scarselli, Marco; Donaldson, Julie G (2009) Constitutive internalization of G protein-coupled receptors and G proteins via clathrin-independent endocytosis. J Biol Chem 284:3577-85
Donaldson, Julie G; Williams, David B (2009) Intracellular assembly and trafficking of MHC class I molecules. Traffic 10:1745-52
Eyster, Craig A; Higginson, Jason D; Huebner, Robert et al. (2009) Discovery of new cargo proteins that enter cells through clathrin-independent endocytosis. Traffic 10:590-9

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