The goals of this research are to develop advanced MRS and imaging techniques and to apply them and other complementary methods to studying brain metabolism, neurotransmission and enzyme activity. MRS allows measurement of neurotransmission of glutamate and GABA in vivo, which play important roles in many major psychiatric diseases including depression and schizophrenia. During 2014-2015, we have made significant progress in the development and applications of novel spectroscopic and imaging techniques for studying metabolism and neurotransmission in vivo in the brain. We have succeeded in routinely obtaining high quality MRS data using a 7 Tesla whole body scanner for measuring glutamate, glutamine and glutathione. This method has been recently validated for its high reproducibility (Reliability of 7T 1 H-MRS measured human prefrontal cortex glutamate, glutamine, and glutathione signals using an adapted echo time optimized PRESS sequence: A between- and within-sessions investigation. Lally N, An L, Banerjee D, Niciu MJ, Luckenbaugh DA, Richards EM, Roiser JP, Shen J, Zarate CA Jr, Nugent AC. J Magn Reson Imaging. 2015 Jun 7. doi: 10.1002/jmri.24970.). We have demonstrated that our 7 Tesla single-shot method can be used to measure the kinetics of 13C label incorporation into amino acid neurotransmitters (Detection of glutamate, glutamine, and glutathione by radiofrequency suppression and echo time optimization at 7 tesla. An L, Li S, Murdoch JB, Araneta MF, Johnson C, Shen J.Magn Reson Med. 2015 Feb;73(2):451-8 and L. An, S. Li, J.B. Murdoch, M.F. Araneta, C. Johnson, and J. Shen, In Vivo Detection of 13C Labeling of Glutamate and Glutamine Using Proton MRS at 7T, International Society for Magnetic Resonance in Medicine annual meeting, 2015.). We have also developed a technique that can reliably extract magnetic resonance spectroscopy signals according to irregularly shaped anatomical boundaries (L. An, and J. Shen, Regularized image-guided spatial localization of heterogeneous compartments for magnetic resonance, Med. Phys., in press.).

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U.S. National Institute of Mental Health
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Evans, Jennifer W; Lally, Níall; An, Li et al. (2018) 7T 1H-MRS in major depressive disorder: a Ketamine Treatment Study. Neuropsychopharmacology 43:1908-1914
Li, Shizhe; An, Li; Duan, Qi et al. (2018) Determining the Rate of Carbonic Anhydrase Reaction in the Human Brain. Sci Rep 8:2328
An, Li; Araneta, Maria Ferraris; Johnson, Christopher et al. (2018) Simultaneous measurement of glutamate, glutamine, GABA, and glutathione by spectral editing without subtraction. Magn Reson Med 80:1776-1786
Marenco, Stefano; Meyer, Christian; van der Veen, Jan Willem et al. (2018) Role of gamma-amino-butyric acid in the dorsal anterior cingulate in age-associated changes in cognition. Neuropsychopharmacology 43:2285-2291
Li, Ningzhi; Li, Shizhe; Shen, Jun (2017) Reconstruction of randomly under-sampled spectra for in vivo13C magnetic resonance spectroscopy. Magn Reson Imaging 37:216-221
Li, Ningzhi; Li, Shizhe; Shen, Jun (2017) High Field In vivo13C Magnetic Resonance Spectroscopy of Brain by Random Radiofrequency Heteronuclear Decoupling and Data Undersampling. Front Phys 5:
van der Veen, Jan Willem; Marenco, Stefano; Berman, Karen F et al. (2017) Retrospective correction of frequency drift in spectral editing: The GABA editing example. NMR Biomed 30:
An, Li; Li, Shizhe; Shen, Jun (2017) Simultaneous determination of metabolite concentrations, T1 and T2 relaxation times. Magn Reson Med 78:2072-2081
Zhang, Yan; An, Li; Shen, Jun (2017) Fast computation of full density matrix of multispin systems for spatially localized in vivo magnetic resonance spectroscopy. Med Phys 44:4169-4178
Zhang, Yan; Shen, Jun (2016) Simultaneous quantification of glutamate and glutamine by J-modulated spectroscopy at 3 Tesla. Magn Reson Med 76:725-32

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