Lesions of the inferior temporal (IT) cortex in humans can result in the clinical syndrome termed prosopagnosia, an inability to recognize familiar faces. Single-cell recordings from IT cortex of monkeys have revealed the existence of neurons that are selectively activated by visual images of faces. More recently, brain imaging studies in both humans and monkeys have demonstrated face-selective regions, in which the fMRI signal evoked by faces is greater compared to that evoked by non-face objects. We have examined the relationship between fMRI-identified face-selective regions in IT cortex of monkeys relative to the selectivity of single neurons, located within vs. outside these regions. Face-preferring neurons were most concentrated in areas corresponding to the fMRI-identified face-selective regions, but were also found, in decreasing concentrations, outside of these regions. Moreover, neurons located within the fMRI-defined face regions showed greater selectivity to faces, compared to those located outside these regions. Thus, fMRI-identified face-selective regions correspond to a high proportion of face-responsive neurons that are highly selective for faces. These findings help to clarify the relationship between fMRI-defined regions and the neuronal processes within them.

 Although face-selective regions have been reported in temporal and prefrontal cortex of both humans and monkeys, the neural circuitry underlying face selectivity remains unclear. To clarify this, we studied the functional connectivity among these face-selective regions in monkeys in the resting, awake state. First, we mapped the face-selective regions by contrasting fMRI activation to images of monkey faces vs. non-face objects. Two face-selective regions in IT cortex were typically found in each hemisphere: the anterior and posterior face patches. Then, the animals underwent ten minutes of resting-state scans. Resting-state average time courses from the anterior and posterior face patches of each hemisphere were used as seeds for functional connectivity analyses. We found that a seed placed in the posterior face patch of one hemisphere correlated with activity in the posterior face patch of the other hemisphere and in the anterior patch, prefrontal face-selective areas and the amygdala of both hemispheres. A seed placed in the anterior face patch showed similar functional connectivity. These results demonstrate that there is a functional network among the face-selective regions, which can be detected by studying the intrinsic, spontaneous fMRI signal fluctuations.

 We have also begun to explore whether the distributed face processing network, revealed under the resting state in healthy individuals, differs in individuals with congenital prosopagnosia (CP), a lifelong deficit in face recognition that occurs despite normal intelligence and sensory experience. We first localized key regions of the face network (fusiform face area, occipital face area, superior temporal sulcus, and anterior temporal lobe) using a face localizer paradigm and used these regions as seeds for a whole brain functional connectivity analysis. This revealed in the controls a set of both posterior and anterior cortical areas whose activity was significantly correlated during rest, reflecting the presence of a face-selective resting state network. However, in CP individuals, the network was compromised, with correlated activity in more anterior regions markedly lower. The results provide further support for the idea that impaired connectivity within the face-processing network may underlie congenital prosopagnosia.

 We had previously shown that facial expressions modulate fMRI activity in face-responsive regions of the monkey's amygdala and visual cortex. Specifically, facial expressions with emotion yield greater activation than neutral faces, a phenomenon known as the valence effect. We next tested the idea that amygdala lesions would eliminate emotional modulatory feedback to the visual cortex, thus disrupting valence effects seen there. We performed selective amygdala lesions in monkeys and then used fMRI to compare the valence effects within the visual cortex in these animals to the effects in normal control animals. Four different facial expressions were tested: neutral, aggressive (open mouth threat), fearful (fear grimace) and appeasing (lipsmack). In controls, faces with emotional expressions relative to neutral faces produced enhanced responses in face-selective regions, as expected. In monkeys with amygdala lesions, although face-selective patches were found in IT cortex, their activity was not modulated by facial expressions. Our data thus demonstrate that the amygdala is the source of the valence modulatory effects seen in the visual cortex. These results make a significant contribution to our understanding of the neural processing of faces with emotional content.

 A number of additional projects have been undertaken in the past year:
 1. Conventional neuroanatomical approaches to study brain circuitry require visualizing transported tracers in post-mortem tissue. We have therefore undertaken the development of a new in vivo tract tracer, by testing a compound that conjugates the conventional neuroanatomical tracer CTB with GdDOTA. We have found that CTB-GdDOTA allows in vivo MR visualization of monosynaptically connected brain circuits, that it is target-specific, bidirectional, very reproducible, and stable over a relatively long period of time. This agent opens the possibility for repetitive, chronic, and longitudinal anatomical studies in monkeys. 2. We have also begun to study anatomical connectivity in monkeys by electrically stimulating a targeted structure and measuring the resultant neuronal activation in functionally connected brain areas with fMRI. To date, we have targeted the basal nucleus of the amygdala and observed preferential activations of face=selective patches in the fundus of the superior temporal sulcus of IT cortex, with little activations of face-selective patches on the lateral bank. Our data thus indicate that modulation of activity in face-selective regions by the amygdala may be specific to some regionsw. 
 3. Recent arguments for face-selective regional homologues between humans and macaques assume common processing strategies. Here, we trained monkeys on a face inversion and composite face illusion task. In the former, subjects responded slower to inverted compared to upright faces. In the latter, subjects again responded slower to the composite illusion, and also had slightly lower accuracy for aligned compared to misaligned faces. These results support increasing evidence that humans and macaques share a common, holistic face-processing system.
 4. One prevailing model of face processing posits that separate networks process either the invariant information of a face, such as identity, or the changeable aspects of a face, such as facial expression. In support of this idea, we found (using a multivoxel pattern analysis approach) that regions responsive to visua motion accurately decoded facial expressions wheres face-selective regions did not. Thus, there appear to be separate networks for facial identity and expression in the monkey temporal cortex.

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Seidlitz, Jakob; Sponheim, Caleb; Glen, Daniel et al. (2018) A population MRI brain template and analysis tools for the macaque. Neuroimage 170:121-131
Zachariou, Valentinos; Del Giacco, Amanda C; Ungerleider, Leslie G et al. (2018) Bottom-up processing of curvilinear visual features is sufficient for animate/inanimate object categorization. J Vis 18:3
Zachariou, Valentinos; Safiullah, Zaid N; Ungerleider, Leslie G (2018) The Fusiform and Occipital Face Areas Can Process a Nonface Category Equivalently to Faces. J Cogn Neurosci 30:1499-1516
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Bell, Andrew H; Summerfield, Christopher; Morin, Elyse L et al. (2016) Encoding of Stimulus Probability in Macaque Inferior Temporal Cortex. Curr Biol 26:2280-90

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