Abstract

The NIA proteomics facility involves extensive collaboration with intramural scientists at different levels of expertise as well as collaboration with different academic institutions. This facility is a multifaceted core facility with technical capabilities that includes HPLC, LC/MS/MS, DIGE 2D gel technology, as well as bioinformatic support with regard to protein isolation, characterization, and large scale proteomics. The unit works directly with IRP investigators, research scientists, post doctoral fellows, and students in training with regard to protein sample preparation and protein characterization.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Scientific Cores Intramural Research (ZIC)
Project #
1ZICAG000576-02
Application #
7969892
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
2
Fiscal Year
2009
Total Cost
$1,198,946
Indirect Cost

  Institution

Name
National Institute on Aging
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Kunin, Margarita; Dmitrieva, Natalia I; Gallazzini, Morgan et al. (2010) Mediator of DNA damage checkpoint 1 (MDC1) contributes to high NaCl-induced activation of the osmoprotective transcription factor TonEBP/OREBP. PLoS One 5:e12108
Wang, Guanghui; Wu, Wells W; Zhang, Zheng et al. (2009) Decoy methods for assessing false positives and false discovery rates in shotgun proteomics. Anal Chem 81:146-59
Yuditskaya, Susan; Tumblin, Ashaunta; Hoehn, Gerard T et al. (2009) Proteomic identification of altered apolipoprotein patterns in pulmonary hypertension and vasculopathy of sickle cell disease. Blood 113:1122-8
Eyster, Craig A; Higginson, Jason D; Huebner, Robert et al. (2009) Discovery of new cargo proteins that enter cells through clathrin-independent endocytosis. Traffic 10:590-9
Looze, Christopher; Yui, David; Leung, Lester et al. (2009) Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein. Biochem Biophys Res Commun 378:433-8
Tsai, Shang-Yi; Hayashi, Teruo; Harvey, Brandon K et al. (2009) Sigma-1 receptors regulate hippocampal dendritic spine formation via a free radical-sensitive mechanism involving Rac1xGTP pathway. Proc Natl Acad Sci U S A 106:22468-73