CHI has produced or contributed to multiple publications in the past year and an additional 10 manuscripts are currently under review or in preparation. We currently manage 29 active collaborations across 11 Institutes and the Clinical Center. CHI onboarded the SomaScan platform which is the only one in the intramural program. Last year, CHI organized a global meeting for SomaScan users to discuss common challenges with the technology. We launched free software developed in house at CHI for analysis of SomaScan data. We onboarding of CyTOF which is the only one on the Bethesda campus. We maintain a standardized biorepository of 20,000 samples for 15 clinical trials. CHI Developed a unique 31 marker (33-parameter) CyTOF PBMC immunophenotyping panel that was utilized by LISB to characterize immune cell landscapes of patients with DOCK11 mutations compared to matched controls, as well as another project discriminating patients with XMEN disease, ALPS, and matched controls from one another(Collaboration with Gregoire Altan-Bonnet/Mike Lenardo/Juan Ravell). We optimized a 34-marker (36-parameter) CyTOF T-cell phenotyping panel that was used to profile bone marrow compartments of healthy controls in a clinical trial (Collaboration with Chris Hourigan). CHI made a ground breaking contribution to standardization of immunophenotyping as part of the Human Immune Phenotyping Consortium (HIPC). CHI continually provides mentoring and service to the intramural community: Our staff regularly teaches FAES classes, and mentors and teaches in the areas of bioinformatics, flow cytometry and advanced genomics.

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2018
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Giudice, Valentina; Wu, Zhijie; Kajigaya, Sachiko et al. (2018) Circulating S100A8 and S100A9 protein levels in plasma of patients with acquired aplastic anemia and myelodysplastic syndromes. Cytokine :
El-Chemaly, Souheil; Cheung, Foo; Kotliarov, Yuri et al. (2018) The Immunome in Two Inherited Forms of Pulmonary Fibrosis. Front Immunol 9:76
Tanaka, Toshiko; Biancotto, Angelique; Moaddel, Ruin et al. (2018) Plasma proteomic signature of age in healthy humans. Aging Cell 17:e12799
Chen, Jinguo; Cheung, Foo; Shi, Rongye et al. (2018) PBMC fixation and processing for Chromium single-cell RNA sequencing. J Transl Med 16:198
Elinoff, Jason M; Chen, Li-Yuan; Dougherty, Edward J et al. (2018) Spironolactone-induced degradation of the TFIIH core complex XPB subunit suppresses NF-?B and AP-1 signalling. Cardiovasc Res 114:65-76
Sanchez, Gina A Montealegre; Reinhardt, Adam; Ramsey, Suzanne et al. (2018) JAK1/2 inhibition with baricitinib in the treatment of autoinflammatory interferonopathies. J Clin Invest 128:3041-3052
Ou, Jingxing; Ball, John M; Luan, Yizhao et al. (2018) iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications. Cell 173:851-863.e16
Hosokawa, Kohei; Kajigaya, Sachiko; Keyvanfar, Keyvan et al. (2017) Whole transcriptome sequencing identifies increased CXCR2 expression in PNH granulocytes. Br J Haematol 177:136-141
Giudice, Valentina; Feng, Xingmin; Kajigaya, Sachiko et al. (2017) Optimization and standardization of fluorescent cell barcoding for multiplexed flow cytometric phenotyping. Cytometry A 91:694-703
Cheung, Foo; Fantoni, Giovanna; Conner, Maria et al. (2017) Web Tool for Navigating and Plotting SomaLogic ADAT Files. J Open Res Softw 5:

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