Abstract

The NCI microscopy core is used consistently by a wide range of NCI researchers with 126 users from over 30 labs so far in 2019 (Jan to July 2019). Forty-three of these were new users that required extensive instrument and image analysis training and supervision. Among the techniques now in use within our CCR microscopy core are methods to visualize live cells in three dimensions over time, including the tracking of cell motion in three-dimensions, in vivo imaging of differentiated induced pluripotent stem cells in 3D spheroids, spontaneous fusion and asymmetric division of cancer stem cells, super-resolution imaging of chromatin modifications in metastatic tumor cells, monitoring protein dynamics in sub-cellular macromolecular structures using FRAP, and multi-position time lapse imaging of live iPS cells with induced gene expression upon cell differentiation. A newly acquired Zeiss Z.1 lightsheet microscope has been used to study tumor cell invasion in live zebrafish, cellular differentiation and organization in developing iPSC organoids, and tumor architecture in cleared mice brains. The number of projects supported represents the diversity of cancer research pursued by CCR investigators.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Scientific Cores Intramural Research (ZIC)
Project #
1ZICBC010858-13
Application #
10015026
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
13
Fiscal Year
2019
Total Cost
Indirect Cost

  Institution

Name
National Cancer Institute Division of Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

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Kaur, Sukhbir; Chang, Tiffany; Singh, Satya P et al. (2014) CD47 signaling regulates the immunosuppressive activity of VEGF in T cells. J Immunol 193:3914-24
Pandey, Gouri Shankar; Yanover, Chen; Miller-Jenkins, Lisa M et al. (2013) Endogenous factor VIII synthesis from the intron 22-inverted F8 locus may modulate the immunogenicity of replacement therapy for hemophilia A. Nat Med 19:1318-24
Kedei, Noemi; Lewin, Nancy E; Géczy, Tamás et al. (2013) Biological profile of the less lipophilic and synthetically more accessible bryostatin 7 closely resembles that of bryostatin 1. ACS Chem Biol 8:767-77
Mineo, Marco; Garfield, Susan H; Taverna, Simona et al. (2012) Exosomes released by K562 chronic myeloid leukemia cells promote angiogenesis in a Src-dependent fashion. Angiogenesis 15:33-45
Geczy, Tamas; Peach, Megan L; El Kazzouli, Saïd et al. (2012) Molecular basis for failure of ""atypical"" C1 domain of Vav1 to bind diacylglycerol/phorbol ester. J Biol Chem 287:13137-58
Xin, Hong-Wu; Hari, Danielle M; Mullinax, John E et al. (2012) Tumor-initiating label-retaining cancer cells in human gastrointestinal cancers undergo asymmetric cell division. Stem Cells 30:591-8
Weng, Meng-Tzu; Lee, Jih-Hsiang; Wei, Shu-Chen et al. (2012) Evolutionarily conserved protein ERH controls CENP-E mRNA splicing and is required for the survival of KRAS mutant cancer cells. Proc Natl Acad Sci U S A 109:E3659-67

Showing the most recent 10 out of 23 publications