The Molecular Cytogenetics Core was involved in the following projects: 1. Detection and delineation of chromosomal rearrangements (by SKY and FISH) generated by a single targeted DNA double strand break in mouse and human cell lines (collaboration with Dr. Aplan). 2. Delineation of complex chromosomal rearrangements and identification of IgH translocation partners by FISH mapping in multiple myeloma cell lines, comprehensive metaphase FISH analyses on advanced multiple myeloma tumors and cell lines (collaboration with Dr. M. Kuehl). 3. Spectral karyotyping of a set of multiple myeloma cell lines and short term cultures, comparison of FISH and SKY data (collaboration with Dr. M. Kuehl and Dr. A. Protopopov). 4. Delineation of complex chromosomal rearrangements in a collection of mesothelioma cell lines (collaboration with Dr. F.Kaye, Dr. G. Klorin, Dr. E. Rozenblum and Dr. O Glebov). 5. Comprehansive characterization of rearrangements present in ovarian tumor samples and cell line derived from tumor by aCGH, gene expression and spectral karyotyping. 6.Identification of IgH translocation partners by FISH mapping in monoclonal gammopathy of undetermined significance (MGAS), smoldering myeloma (SMM) and multiple myeloma (MM) patient samples (collaboration with Dr. A. Zingone, Dr. M. Kuehl and Dr.K. Lindgren).

National Institute of Health (NIH)
National Cancer Institute (NCI)
Scientific Cores Intramural Research (ZIC)
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National Cancer Institute Division of Basic Sciences
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Affer, Maurizio; Chesi, Marta; Chen, Wei-Dong G et al. (2014) Promiscuous MYC locus rearrangements hijack enhancers but mostly super-enhancers to dysregulate MYC expression in multiple myeloma. Leukemia 28:1725-1735
Kim, Gina Y; Gabrea, Ana; Demchenko, Yulia N et al. (2014) Complex IGH rearrangements in multiple myeloma: Frequent detection discrepancies among three different probe sets. Genes Chromosomes Cancer 53:467-74
Klorin, Geula; Rozenblum, Ester; Glebov, Oleg et al. (2013) Integrated high-resolution array CGH and SKY analysis of homozygous deletions and other genomic alterations present in malignant mesothelioma cell lines. Cancer Genet 206:191-205
Roschke, Anna V; Rozenblum, Ester (2013) Multi-layered cancer chromosomal instability phenotype. Front Oncol 3:302
Roschke, Anna V; Kirsch, Ilan R (2010) Targeting Karyotypic Complexity and Chromosomal Instability of Cancer Cells. Curr Drug Targets :
Cheng, Yue; Zhang, Zhenhua; Keenan, Bridget et al. (2010) Efficient repair of DNA double-strand breaks in malignant cells with structural instability. Mutat Res 683:115-22
Roschke, Anna V; Kirsch, Ilan R (2010) Targeting karyotypic complexity and chromosomal instability of cancer cells. Curr Drug Targets 11:1341-50
Roschke, Anna V; Glebov, Oleg K; Lababidi, Samir et al. (2008) Chromosomal instability is associated with higher expression of genes implicated in epithelial-mesenchymal transition, cancer invasiveness, and metastasis and with lower expression of genes involved in cell cycle checkpoints, DNA repair, and chromatin mai Neoplasia 10:1222-30
Gabrea, Ana; Martelli, Maria Luisa; Qi, Ying et al. (2008) Secondary genomic rearrangements involving immunoglobulin or MYC loci show similar prevalences in hyperdiploid and nonhyperdiploid myeloma tumors. Genes Chromosomes Cancer 47:573-90