Abstract

The Core has two objectives. First, its mission is to provide state-of-the-art light microscopy instrumentation for the Lab of Receptor Biology and Gene Expression. Second, the Core provides support to anyone in the CCR who requires assistance with fluorescence imaging. Present work in the Core revolves around four different general techniques: structural studies such as colocalization of different fluorescent markers, time-lapse analysis to follow how cellular components change over time, fluorescence recovery after photobleaching to measure mobility and binding of cell molecules and fluorescence resonance energy transfer to measure molecular interactions in live or fixed specimens. The following techniques are currently being adapted for the needs of the Core users: Number and Brightness quantification of the diffusion and binding rates of cell molecules and Super resolution imaging (PALM/SIM/dSTORM). The Core is building a Single Molecule /PALM /dSTORM microscope for the further development of single molecule tracking and molecule counting by superresolution.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Scientific Cores Intramural Research (ZIC)
Project #
1ZICBC011574-01
Application #
8938495
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2014
Total Cost
Indirect Cost

  Institution

Name
Basic Sciences
Department
Type
DUNS #
City
State
Country
Zip Code

  Publications

Kannan, Ramakrishnan; Song, Jeong-Kuen; Karpova, Tatiana et al. (2017) The Abl pathway bifurcates to balance Enabled and Rac signaling in axon patterning in Drosophila. Development 144:487-498
Torabi, Keyvan; Wangsa, Darawalee; Ponsa, Immaculada et al. (2017) Transcription-dependent radial distribution of TCF7L2 regulated genes in chromosome territories. Chromosoma 126:655-667
Shrestha, Roshan L; Ahn, Grace S; Staples, Mae I et al. (2017) Mislocalization of centromeric histone H3 variant CENP-A contributes to chromosomal instability (CIN) in human cells. Oncotarget 8:46781-46800
Vargas-Inchaustegui, Diego A; Demers, Andrew; Shaw, Julia M et al. (2016) Vaccine Induction of Lymph Node-Resident Simian Immunodeficiency Virus Env-Specific T Follicular Helper Cells in Rhesus Macaques. J Immunol 196:1700-10
Kim, Jeongkyu; Sturgill, David; Tran, Andy D et al. (2016) Controlled DNA double-strand break induction in mice reveals post-damage transcriptome stability. Nucleic Acids Res 44:e64
Kononenko, Artem V; Lee, Nicholas C O; Liskovykh, Mikhail et al. (2015) Generation of a conditionally self-eliminating HAC gene delivery vector through incorporation of a tTAVP64 expression cassette. Nucleic Acids Res 43:e57
Glancy, Brian; Hartnell, Lisa M; Malide, Daniela et al. (2015) Mitochondrial reticulum for cellular energy distribution in muscle. Nature 523:617-20
Athwal, Rajbir K; Walkiewicz, Marcin P; Baek, Songjoon et al. (2015) CENP-A nucleosomes localize to transcription factor hotspots and subtelomeric sites in human cancer cells. Epigenetics Chromatin 8:2
Stavreva, Diana A; Coulon, Antoine; Baek, Songjoon et al. (2015) Dynamics of chromatin accessibility and long-range interactions in response to glucocorticoid pulsing. Genome Res 25:845-57
Presman, Diego M; Ogara, M Florencia; Stortz, Martín et al. (2014) Live cell imaging unveils multiple domain requirements for in vivo dimerization of the glucocorticoid receptor. PLoS Biol 12:e1001813

Showing the most recent 10 out of 19 publications