The Experimental Pathology Laboratory (EPL) provides research support to members of the Laboratory of Pathology. Specifically, the EPL provides via simple request: tissue processing, and staining, immunohistohistochemistry, in situ hybridization and whole slide imaging on a request basis. More complex, non-menu activities supported include development of new immunohistochemical assays, tissue microarray construction, laser capture microdissection, image analysis and 3D-printer services. Long-term research efforts in the EPL, in support of translational pathology include: developments of new hardware for the construction of tissue microarrays, the development of a tissue microarray instrument for frozen tissue, refinement of technologies for tissue micro-dissection. Additional efforts in the EPL focus on tissue preservation and storage, with efforts in the development of non-formaldehyde containing tissue fixatives, refinement to tissue impregnation systems to prevent tissue degradation and development of methods for freezing and storing tissue that prevent degradation. The EPL was initiated in FY15, and has filed to invention reports in this fiscal year, both of which will be filed as preliminary patent applications.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Scientific Cores Intramural Research (ZIC)
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Basic Sciences
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Chung, Joon-Yong; Song, Joon Seon; Ylaya, Kris et al. (2018) Histomorphological and Molecular Assessments of the Fixation Times Comparing Formalin and Ethanol-Based Fixatives. J Histochem Cytochem 66:121-135
Hewitt, Stephen M (2017) Formulation and pH of the Buffered Ethanol Fixative BE70. J Histochem Cytochem 65:251-252
Choi, Chel Hun; Chung, Joon-Yong; Kim, Jae-Hoon et al. (2016) Expression of fibroblast growth factor receptor family members is associated with prognosis in early stage cervical cancer patients. J Transl Med 14:124
Chung, Joon-Yong; Cho, Hanbyoul; Hewitt, Stephen M (2016) The paraffin-embedded RNA metric (PERM) for RNA isolated from formalin-fixed, paraffin-embedded tissue. Biotechniques 60:239-44
Chung, Joon-Yong; Choi, Jiyeon; Sears, John D et al. (2016) A melanin-bleaching methodology for molecular and histopathological analysis of formalin-fixed paraffin-embedded tissue. Lab Invest 96:1116-27
Perry, Candice; Chung, Joon-Yong; Ylaya, Kris et al. (2016) A Buffered Alcohol-Based Fixative for Histomorphologic and Molecular Applications. J Histochem Cytochem 64:425-40
Choi, Chel Hun; Chung, Joon-Yong; Cho, Hanbyoul et al. (2015) Prognostic Significance of AMP-Dependent Kinase Alpha Expression in Cervical Cancer. Pathobiology 82:203-11
Treanor, Darren; Gallas, Brandon D; Gavrielides, Marios A et al. (2015) Evaluating whole slide imaging: A working group opportunity. J Pathol Inform 6:4
Chung, Joon-Yong; Hewitt, Stephen M (2015) A well-based reverse-phase protein array of formalin-fixed paraffin-embedded tissue. Methods Mol Biol 1312:129-39
Hewitt, Stephen M (2015) The evolution of pre-analytic factors in Anatomic Pathology. Recent Results Cancer Res 199:27-34

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