We have the methods and facilities to package and purify both adeno-associated viral (AAV) vectors and lentiviral vectors (LV) for gene delivery studies. We have been making AAV vectors for over 6 years and have recently begun working with lentiviral vectors. We are routinely generate new vectors both for our lab and for collaborations. The vectors are designed primarily for studies focused on studying the the molecular mechanisms of addiction and neurodegeneration. For example, we have well characterized and novel neurotrophic factors such as brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), bone morphogenetic protein 7 (BMP7), mesencephalic astrocyte-derived neurotrophic factor (MANF) and conserved dopaminerigic neurotrophic factor (CDNF). We also work with neuromodulatory genes such as neurotransmitter tranporters and receptors. Our vectors are available for intramural and extramural collaborators.

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Scientific Cores Intramural Research (ZIC)
Project #
1ZICDA000555-01
Application #
7970062
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
1
Fiscal Year
2009
Total Cost
$282,953
Indirect Cost
Name
National Institute on Drug Abuse
Department
Type
DUNS #
City
State
Country
Zip Code
Hamra, F Kent; Richie, Christopher T; Harvey, Brandon K (2017) Long Evans rat spermatogonial lines are effective germline vectors for transgenic rat production. Transgenic Res 26:477-489
Wallace, Veronica J; Cimbro, Raffaello; Rubio, F Javier et al. (2017) Neurons Internalize Functionalized Micron-Sized Silicon Dioxide Microspheres. Cell Mol Neurobiol 37:1487-1499
Sharpe, Melissa J; Marchant, Nathan J; Whitaker, Leslie R et al. (2017) Lateral Hypothalamic GABAergic Neurons Encode Reward Predictions that Are Relayed to the Ventral Tegmental Area to Regulate Learning. Curr Biol 27:2089-2100.e5
Howard, Douglas B; Harvey, Brandon K (2017) Assaying the Stability and Inactivation of AAV Serotype 1 Vectors. Hum Gene Ther Methods 28:39-48
Wires, Emily S; Henderson, Mark J; Yan, Xiaokang et al. (2017) Longitudinal monitoring of Gaussia and Nano luciferase activities to concurrently assess ER calcium homeostasis and ER stress in vivo. PLoS One 12:e0175481
Richie, Christopher T; Whitaker, Leslie R; Whitaker, Keith W et al. (2017) Near-infrared fluorescent protein iRFP713 as a reporter protein for optogenetic vectors, a transgenic Cre-reporter rat, and other neuronal studies. J Neurosci Methods 284:1-14
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Marchant, Nathan J; Campbell, Erin J; Whitaker, Leslie R et al. (2016) Role of Ventral Subiculum in Context-Induced Relapse to Alcohol Seeking after Punishment-Imposed Abstinence. J Neurosci 36:3281-94
Lee, Chun-Ting; Chen, Jia; Kindberg, Abigail A et al. (2016) CYP3A5 Mediates Effects of Cocaine on Human Neocorticogenesis: Studies using an In Vitro 3D Self-Organized hPSC Model with a Single Cortex-Like Unit. Neuropsychopharmacology :
Kuo, Chi-Chung; Shen, Hui; Harvey, Brandon K et al. (2016) Differential modulation of methamphetamine-mediated behavioral sensitization by overexpression of Mu opioid receptors in nucleus accumbens and ventral tegmental area. Psychopharmacology (Berl) 233:661-72

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