We have used conventional chemical fixation, dehydration, embedding and staining procedures to carry out studies on several fundamental biological processes, including membrane fusion, macroautophagy, and motility. We established a number of collaborative projects with multiple institutes across NIH. For example, with NIA we performed studies on the neuroprotective mechanism of NAM, the NAD+ precursor, and its effects on the autophagy-system in neurons exposed to oxidative stress;with NIDCR we examined mucin-type O-linked glycosylation in the structures of digestive system during development in Drosophila;and with NHLBI we investigated the process of selective degradation of mitochondria by using Drosophila cell lines. We also collaborated with a group in the Johns Hopkins University School of Medicine to use a high-pressure freezing/freeze-substitution method, the most useful and powerful cryofixation method, to study dynamic rearrangements of the actin cytoskeleton in Drosophila. The findings were published in the May issue of Developmental Cell. In addition, quite a few projects involving the characterization of nanoparticles and drug delivery mechanisms were performed by using electron microscopy techniques, including negative staining methods. Investigators within the IRPs of NIBIB, NIDDK and NINDS initiated most of these projects.

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National Institute of Biomedical Imaging and Bioengineering
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Bryant Jr, L Henry; Kim, Saejeong J; Hobson, Matthew et al. (2017) Physicochemical characterization of ferumoxytol, heparin and protamine nanocomplexes for improved magnetic labeling of stem cells. Nanomedicine 13:503-513
Song, Jibin; Wu, Binghui; Zhou, Zijian et al. (2017) Double-Layered Plasmonic-Magnetic Vesicles by Self-Assembly of Janus Amphiphilic Gold-Iron(II,III) Oxide Nanoparticles. Angew Chem Int Ed Engl 56:8110-8114
Lu, Nan; Huang, Peng; Fan, Wenpei et al. (2017) Tri-stimuli-responsive biodegradable theranostics for mild hyperthermia enhanced chemotherapy. Biomaterials 126:39-48
Lin, Jing; Wang, Min; Hu, Hao et al. (2016) Multimodal-Imaging-Guided Cancer Phototherapy by Versatile Biomimetic Theranostics with UV and ?-Irradiation Protection. Adv Mater 28:3273-9
Chen, Zhe; Qi, Yun; French, Stephanie et al. (2015) Genetic mosaic analysis of a deleterious mitochondrial DNA mutation in Drosophila reveals novel aspects of mitochondrial regulation and function. Mol Biol Cell 26:674-84
Shomorony, A; Pfeifer, C R; Aronova, M A et al. (2015) Combining quantitative 2D and 3D image analysis in the serial block face SEM: application to secretory organelles of pancreatic islet cells. J Microsc 259:155-164
Wang, Zhe; Wang, Yu; Wang, Zhiyong et al. (2015) Polymeric Nanovehicle Regulated Spatiotemporal Real-Time Imaging of the Differentiation Dynamics of Transplanted Neural Stem Cells after Traumatic Brain Injury. ACS Nano 9:6683-95
Pfeifer, C R; Shomorony, A; Aronova, M A et al. (2015) Quantitative analysis of mouse pancreatic islet architecture by serial block-face SEM. J Struct Biol 189:44-52
Chen, Richard J; Zhang, Guofeng; Garfield, Susan H et al. (2015) Variations in Glycogen Synthesis in Human Pluripotent Stem Cells with Altered Pluripotent States. PLoS One 10:e0142554
Qi, Y; Liu, H; Daniels, M P et al. (2015) Loss of Drosophila i-AAA protease, dYME1L, causes abnormal mitochondria and apoptotic degeneration. Cell Death Differ :

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