The NIEHS knock out core (KOC) is a servicing core facility with some research opportunity. Each project in the core is a long-term commitment from start to finish, and several projects are at different stages of completion in the core at any given time of the year. The core works very closely with the investigator since the inception of the project, which typically starts with meeting the PI's group for developing the targeting strategy and continues until the mutant mouse is made. KOC helps investigators to generate the mutant mouse customized to their research needs;including traditional knockouts, conditional knockout and knock-ins. It also provides assistance to establish and culturing the mutant cells for in vitro studies, along with dissecting the early stage embryos and help establishing the genotyping strategies.

Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
2011
Total Cost
$896,571
Indirect Cost
City
State
Country
Zip Code
Edin, Matthew L; Hamedani, Behin Gholipour; Gruzdev, Artiom et al. (2018) Epoxide hydrolase 1 (EPHX1) hydrolyzes epoxyeicosanoids and impairs cardiac recovery after ischemia. J Biol Chem 293:3281-3292
Martin, Negin P; Myers, Page; Goulding, Eugenia et al. (2018) En masse lentiviral gene delivery to mouse fertilized eggs via laser perforation of zona pellucida. Transgenic Res 27:39-49
Scott, Gregory J; Gruzdev, Artiom; Hagler, Thomas B et al. (2018) Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates. J Vis Exp :
Ungewitter, Erica K; Rotgers, Emmi; Kang, Hong Soon et al. (2018) Loss of Glis3 causes dysregulation of retrotransposon silencing and germ cell demise in fetal mouse testis. Sci Rep 8:9662
Stefkovich, Megan L; Arao, Yukitomo; Hamilton, Katherine J et al. (2018) Experimental models for evaluating non-genomic estrogen signaling. Steroids 133:34-37
Mesev, Emily V; Miller, David S; Cannon, Ronald E (2017) Ceramide 1-Phosphate Increases P-Glycoprotein Transport Activity at the Blood-Brain Barrier via Prostaglandin E2 Signaling. Mol Pharmacol 91:373-382
Pan, Haichun; Zhang, Honghao; Abraham, Ponnu et al. (2017) BmpR1A is a major type 1 BMP receptor for BMP-Smad signaling during skull development. Dev Biol 429:260-270
Garcia, Victor; Gilani, Ankit; Shkolnik, Brian et al. (2017) 20-HETE Signals Through G-Protein-Coupled Receptor GPR75 (Gq) to Affect Vascular Function and Trigger Hypertension. Circ Res 120:1776-1788
Hofer, M; Hoferová, Z; Dušek, L et al. (2017) Hematological profile of untreated or ionizing radiation-exposed cyclooxygenase-2-deficient mice. Physiol Res 66:673-676
Nakano, Hideki; Lyons-Cohen, Miranda R; Whitehead, Gregory S et al. (2017) Distinct functions of CXCR4, CCR2, and CX3CR1 direct dendritic cell precursors from the bone marrow to the lung. J Leukoc Biol 101:1143-1153

Showing the most recent 10 out of 56 publications