The NHGRI Microarray Core provides resources, cost-effective and time-efficient services, advice and technical support to meet the needs of NHGRI, NINDS, and NIMH investigators related to genomics research. The services primarily focus on gene expression and genotyping technologies. Researchers utilize the expertise, protocols and instrumentation available in the Core laboratory. The Core adapts readily as the new technologies emerge and aids in both performing laboratory services as well as experimental design. In the past year gene expression services were provided using mostly commercial (Affymetrix, Agilent and Illumina) arrays. In-house arrays, however, were available for zebrafish expression studies. The Core provided expression analysis using >1600 arrays (Affymetrix, Agilent, Illumina and In-house oligos chips). The investigators utilized the expression services (total RNA and microRNAs) to gain insight into a variety of human disease conditions, including Niemann-Pick disease, type C (NPC), melanoma, endometrial cancer, Progeria syndrome, type II diabetes, blood malignancies, and Gaucher disease. We have also been involved in expression studies of immune cell characterization and normal skin bacterial flora. In addition, studies of disease models and developmental changes in the mouse have been done. Studies include mouse lung sensitivity due to to dust mites, and glial cell migration. With regard to human disease a variety of studies have been done including those aimed at understanding expression profiles associated with schizophrenia, autism and other behavioral phenotypes. Finally, zebrafish germ layer development, sex differentiation, and hair cell regeneration have also studied this year employing the gene expression technologies of the Array Core. SNP-based microarray technologies from Affymetrix and Illumina were used by the Core to provide genotyping services critical for genome wide association (GWAS) studies. These services were applied for a variety of studies related to human neuronal diseases, cancer, and diabetes. In addition, the core optimized SNP chip technologies related to the canine model, and as a result several studies were undertaken to find genes that control morphologic variation across the species, as well as breed susceptibility to various forms of cancer including gastric, histocytic sarcoma, transitional cell carcinoma of the bladder, and squamous cell carcinoma. The dog studies alone involved running over 1,500 SNP chips. In summary, the NHGRI Array Core has met the needs to multiple investigators in three Institutes for both expression and SNP-based genotyping studies. A number of publications arose from this work and Core staff have been listed as authors on nine and cited in the acknowledgements of several others.

National Institute of Health (NIH)
National Human Genome Research Institute (NHGRI)
Scientific Cores Intramural Research (ZIC)
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Yu, Yong; Schleich, Kolja; Yue, Bin et al. (2018) Targeting the Senescence-Overriding Cooperative Activity of Structurally Unrelated H3K9 Demethylases in Melanoma. Cancer Cell 33:322-336.e8
Elkahloun, Abdel G; Rodriguez, Yara; Alaiyed, Seham et al. (2018) Telmisartan Protects a Microglia Cell Line from LPS Injury Beyond AT1 Receptor Blockade or PPAR? Activation. Mol Neurobiol :
Yu, Yong; Schleich, Kolja; Yue, Bin et al. (2018) Targeting the Senescence-Overriding Cooperative Activity of Structurally Unrelated H3K9 Demethylases in Melanoma. Cancer Cell 33:785
Nordlinger, Alice; Dror, Shani; Elkahloun, Abdel et al. (2018) Mutated MITF-E87R in Melanoma Enhances Tumor Progression via S100A4. J Invest Dermatol 138:2216-2223
Qutob, Nouar; Masuho, Ikuo; Alon, Michal et al. (2018) RGS7 is recurrently mutated in melanoma and promotes migration and invasion of human cancer cells. Sci Rep 8:653
Kaur, Sukhbir; Elkahloun, Abdel G; Arakelyan, Anush et al. (2018) CD63, MHC class 1, and CD47 identify subsets of extracellular vesicles containing distinct populations of noncoding RNAs. Sci Rep 8:2577
Li, Zejuan; Huang, Hao; Chen, Ping et al. (2018) Publisher Correction: miR-196b directly targets both HOXA9/MEIS1 oncogenes and FAS tumour suppressor in MLL-rearranged leukaemia. Nat Commun 9:16192
Johnson, Tory P; Tyagi, Richa; Lee, Paul R et al. (2017) Nodding syndrome may be an autoimmune reaction to the parasitic worm Onchocerca volvulus. Sci Transl Med 9:
Lehmann, Michael L; Weigel, Thaddeus K; Elkahloun, Abdel G et al. (2017) Chronic social defeat reduces myelination in the mouse medial prefrontal cortex. Sci Rep 7:46548
Pemov, A; Li, H; Patidar, R et al. (2017) The primacy of NF1 loss as the driver of tumorigenesis in neurofibromatosis type 1-associated plexiform neurofibromas. Oncogene 36:3168-3177

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