Collaborative and support oriented work has over the past year had an impact in the study, and on the understanding, of a spectrum of diseases and disease processes such as cardiovascular disease (atherosclerosis), inflammatory respiratory disease, obesity and type II diabetes, inflammatory and immune-mediated renal disease, gene expression and gene regulation, axial skeletal development, cancer and transgenerational effects of diet restriction. Additionally, associations between exacerbation of chronic progressive nephropathy in subchronic studies and increased incidence of renal tumors in subsequent 2-year studies was examined for male rats. In addition, the mouse phenotyping group has assisted with the phenotyping a few adult genetically engineered mice projects. While the focus of services provided by this facility has been on the histological assessment with embryonic lethal phenotypes, additional services have been provided in assisting with special necropsy techniques and special applications with regards to RNA isolation. The laser microdissection group has worked with investigators to establish successful protocols for the isolation of protein (western blots and proteomic analysis), RNA from frozen and more recently from laser-microdissected, paraffin-embedded tissue (quantitative RT-PCR) and DNA (embryo genotyping) from laser-microdissected tissue samples. The immunohistochemistry group has worked with investigators to establish immunostaining protocols for a variety of unique tissue markers.

Project Start
Project End
Budget Start
Budget End
Support Year
4
Fiscal Year
2011
Total Cost
$7,430,346
Indirect Cost
City
State
Country
Zip Code
Edin, Matthew L; Wang, Zhongjing; Bradbury, J Alyce et al. (2011) Endothelial expression of human cytochrome P450 epoxygenase CYP2C8 increases susceptibility to ischemia-reperfusion injury in isolated mouse heart. FASEB J 25:3436-47
Thomas, Abe C; Nouls, John C; Driehuys, Bastiaan et al. (2011) Ventilation defects observed with hyperpolarized 3He magnetic resonance imaging in a mouse model of acute lung injury. Am J Respir Cell Mol Biol 44:648-54
Palmer, Scott M; Flake, Gordon P; Kelly, Fran L et al. (2011) Severe airway epithelial injury, aberrant repair and bronchiolitis obliterans develops after diacetyl instillation in rats. PLoS One 6:e17644
Deng, Yangmei; Edin, Matthew L; Theken, Katherine N et al. (2011) Endothelial CYP epoxygenase overexpression and soluble epoxide hydrolase disruption attenuate acute vascular inflammatory responses in mice. FASEB J 25:703-13
Adams, E Terence; Auerbach, Scott; Blackshear, Pamela E et al. (2011) Proceedings of the 2010 National Toxicology Program Satellite Symposium. Toxicol Pathol 39:240-66
Lee, Craig R; Imig, John D; Edin, Matthew L et al. (2010) Endothelial expression of human cytochrome P450 epoxygenases lowers blood pressure and attenuates hypertension-induced renal injury in mice. FASEB J 24:3770-81
Witt, Kristine L; Malarkey, David E; Hobbs, Cheryl A et al. (2010) No increases in biomarkers of genetic damage or pathological changes in heart and brain tissues in male rats administered methylphenidate hydrochloride (Ritalin) for 28 days. Environ Mol Mutagen 51:80-8
Crawford, Laura Wilding; Foley, Julie F; Elmore, Susan A (2010) Histology atlas of the developing mouse hepatobiliary system with emphasis on embryonic days 9.5-18.5. Toxicol Pathol 38:872-906
Shim, Minsub; Foley, Julie; Anna, Colleen et al. (2010) Embryonic expression of cyclooxygenase-2 causes malformations in axial skeleton. J Biol Chem 285:16206-17
Ghanayem, Burhan I; Bai, Re; Kissling, Grace E et al. (2010) Diet-induced obesity in male mice is associated with reduced fertility and potentiation of acrylamide-induced reproductive toxicity. Biol Reprod 82:96-104

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