In collaboration with the laboratory of Dr, Flemming Hansen, Technical University of Denmark, we have developed a fully automated cell recognition and fluorescent focus-measuring program in the form of a macro within the image analysis program Image Pro Plus. This has been very successful in the basic tasks of detecting fluorescent foci formed by plasmids and specific chromosomal loci in cells of the bacterium Escherichia coli. As our needs for analysis of fluorescent images become more demanding, we continue to develop and adapt this system to meet them. We are developing an analytical tool Inspect that facilitates the analysis of images that have two color fluorescence information. In conjunction with the data collection programs, we are developing an array of spreadsheet programs to analyze and portray the data.
|Sengupta, Manjistha; Nielsen, Henrik Jorck; Youngren, Brenda et al. (2010) P1 plasmid segregation: accurate redistribution by dynamic plasmid pairing and separation. J Bacteriol 192:1175-83|
|Dabrazhynetskaya, Alena; Brendler, Therese; Ji, Xinhua et al. (2009) Switching protein-DNA recognition specificity by single-amino-acid substitutions in the P1 par family of plasmid partition elements. J Bacteriol 191:1126-31|