The ultimate goal of the Human Genome Program is the determination of the nucleotide sequence of the human genome. the five year plan for this program includes the following explicit goals: the construction of high resolution genetic linkage maps, the development of physical maps, the determination of the complete nucleotide sequence of the human genome and the genomes of selected organisms, and the development of appropriate new technologies to achieve these goals. Critical to the success of these efforts is the development of new technologies to reduce costs and to improve throughput. The proposed GESTEC grant will focus of applications of DNA oligonucleotide arrays to the Human Genome project. Sequencing and mapping components will address the development of chip based de novo sequencing, resequencing, and sequence checking and physical, genetic, and functional mapping leading to a pilot scale project. The technology development project will address the production of chips and the development of instrumentation and software specific to the chip applications described above. Practical applications of the DNA chip technology will be scaled up and made available to the User Center, which will provide an opportunity for outside researchers to use the DNA chips and serve as a technology transfer facility. Successful completion of the proposed program will provide significant new capabilities for genomic sequencing and mapping.

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Research Program Projects (P01)
Project #
5P01HG001323-03
Application #
2519136
Study Section
Special Emphasis Panel (SRC (06))
Project Start
1995-09-01
Project End
1999-08-31
Budget Start
1997-09-30
Budget End
1999-08-31
Support Year
3
Fiscal Year
1997
Total Cost
Indirect Cost
Name
Affymetrix, Inc.
Department
Type
DUNS #
804682573
City
Santa Clara
State
CA
Country
United States
Zip Code
95051
Lee, Albert; Henras, Anthony K; Chanfreau, Guillaume (2005) Multiple RNA surveillance pathways limit aberrant expression of iron uptake mRNAs and prevent iron toxicity in S. cerevisiae. Mol Cell 19:39-51
Danin-Kreiselman, Michal; Lee, Chrissie Young; Chanfreau, Guillaume (2003) RNAse III-mediated degradation of unspliced pre-mRNAs and lariat introns. Mol Cell 11:1279-89
Patti, Mary Elizabeth; Butte, Atul J; Crunkhorn, Sarah et al. (2003) Coordinated reduction of genes of oxidative metabolism in humans with insulin resistance and diabetes: Potential role of PGC1 and NRF1. Proc Natl Acad Sci U S A 100:8466-71
Zheng, Z; Venkatapathy, S; Rao, G et al. (2002) Expression profiling of B cell chronic lymphocytic leukemia suggests deficient CD1-mediated immunity, polarized cytokine response, altered adhesion and increased intracellular protein transport and processing of leukemic cells. Leukemia 16:2429-37
Schulze, A; Lehmann, K; Jefferies, H B et al. (2001) Analysis of the transcriptional program induced by Raf in epithelial cells. Genes Dev 15:981-94
Soulez, M; Parker, M G (2001) Identification of novel oestrogen receptor target genes in human ZR75-1 breast cancer cells by expression profiling. J Mol Endocrinol 27:259-74
Nadler, S T; Stoehr, J P; Schueler, K L et al. (2000) The expression of adipogenic genes is decreased in obesity and diabetes mellitus. Proc Natl Acad Sci U S A 97:11371-6
Hacia, J G; Fan, J B; Ryder, O et al. (1999) Determination of ancestral alleles for human single-nucleotide polymorphisms using high-density oligonucleotide arrays. Nat Genet 22:164-7
Gentalen, E; Chee, M (1999) A novel method for determining linkage between DNA sequences: hybridization to paired probe arrays. Nucleic Acids Res 27:1485-91
Sapolsky, R J; Hsie, L; Berno, A et al. (1999) High-throughput polymorphism screening and genotyping with high-density oligonucleotide arrays. Genet Anal 14:187-92

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