instmctions); The Mouse Behavioral Assessment and Breeding Core is an extension of a core in the previously supported program. The Core has a number of specific tasks that support all of the projects in the program. The specific tasks undertaken by the experienced Core staff are the following: a) behavioral assessment of wake and sleep stages in mice using high throughput video assessment of behavior. In the previous period of funding we developed a novel methodology to assess wake and stages of sleep from video analysis;2) recording of wake and sleep using electroencephalogram and electromyogram. This involves implantation of electrodes and careful scoring of records. Core staff also perform spectral analysis of the EEG signal;3) these studies generate a considerable amount of behavioral data. Thus, the Core also does data reduction and, using customized software we have developed, provides summary data to investigators in an easy to access format;4) this new program of research now involves breeding and genotyping of a number of specific transgenic mice. It is proposed that this is best done by a central facility and therefore this Core now provides breeding of mice and genotyping. While these are ongoing and enhanced services that the Core will provide, the Core is also engaged in innovative activities. First, Core staff will conduct a study of changes in sleep/wake across the lifespan in C57BL/6NIA mice. These mice are used in Projects 01 and 02. Thus, these data will inform the science of these two projects. The Core is also addressing the capability to create new transgenic mice. As a first step, we proposed to create conditional transgenics where expression of Homer l a can be increased in older animals. This is an immediate future direction for Project 02. Finally, the Core will work to extend, in collaboration with staff in Core C, the video-based technology so that wake and stages of sleep can be assessed in individual mice among a group of mice that are raised together.

Public Health Relevance

(See Instmctions): Mice are a powerful model system to study sleep and aging effects. Mice show the same changes in wake and sleep as humans. This Core provides the ability to study sleep and wake in mice of all ages to provide the behavioral assessments needed for this program of research. It also will create mutant mice that provide the capability to study mice with alteration in a particular gene and be responsible for mouse breeding.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Research Program Projects (P01)
Project #
5P01AG017628-12
Application #
8642129
Study Section
Special Emphasis Panel (ZAG1-ZIJ-5)
Project Start
Project End
Budget Start
2014-04-01
Budget End
2015-03-31
Support Year
12
Fiscal Year
2014
Total Cost
$322,712
Indirect Cost
$89,097
Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Zimmerman, John E; Chan, May T; Lenz, Olivia T et al. (2017) Glutamate Is a Wake-Active Neurotransmitter in Drosophila melanogaster. Sleep 40:
Anafi, Ron C; Francey, Lauren J; Hogenesch, John B et al. (2017) CYCLOPS reveals human transcriptional rhythms in health and disease. Proc Natl Acad Sci U S A 114:5312-5317
Gerstner, Jason R; Lenz, Olivia; Vanderheyden, William M et al. (2017) Amyloid-? induces sleep fragmentation that is rescued by fatty acid binding proteins in Drosophila. J Neurosci Res 95:1548-1564
Havekes, Robbert; Abel, Ted (2017) The tired hippocampus: the molecular impact of sleep deprivation on hippocampal function. Curr Opin Neurobiol 44:13-19
Morgan, Andrew P; Gatti, Daniel M; Najarian, Maya L et al. (2017) Structural Variation Shapes the Landscape of Recombination in Mouse. Genetics 206:603-619
Gardner, Benjamin; Strus, Ewa; Meng, Qing Cheng et al. (2016) Sleep Homeostasis and General Anesthesia: Are Fruit Flies Well Rested after Emergence from Propofol? Anesthesiology 124:404-16
Havekes, Robbert; Park, Alan J; Tolentino, Rosa E et al. (2016) Compartmentalized PDE4A5 Signaling Impairs Hippocampal Synaptic Plasticity and Long-Term Memory. J Neurosci 36:8936-46
Havekes, Robbert; Park, Alan J; Tudor, Jennifer C et al. (2016) Sleep deprivation causes memory deficits by negatively impacting neuronal connectivity in hippocampal area CA1. Elife 5:
Tudor, Jennifer C; Davis, Emily J; Peixoto, Lucia et al. (2016) Sleep deprivation impairs memory by attenuating mTORC1-dependent protein synthesis. Sci Signal 9:ra41
Perron, Isaac J; Pack, Allan I; Veasey, Sigrid (2015) Diet/Energy Balance Affect Sleep and Wakefulness Independent of Body Weight. Sleep 38:1893-903

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