The Analytical Core will provide a variety of specialized analytical services for the investigators of the Program Project to assist in the conduct ofthe molecular, cellular and in vivo studies. The Analytical Core will be a collaborative effort between units at the University of California, San Diego under Dr. Joseph Witztum and one at the Salk Institute under the direction of Dr. Ronald Evans. The overall goal of the Analytical Core is to take advantage of specialized resources and expertise to provide investigators with selected core services that will assist in the research mission of each Project.
The Aims of this Core are:
Specific Aim 1 : To provide high-throughput nano-scale quantitative PCR analysis (Nano-QPCR) profiling of gene signatures and pathways. Here we describe the equipment and operation procedures for high-throughput nano-scale quantitative, real-time, reverse-transcription PCR (Nano-QPCR) for surveying SNP genotyping and profiling the expression of particular transcripts in samples provided by PPG investigators.
Specific Aim 2 : To provide high throughput, high sensitivity analysis of cytokines and chemokines with the use of Luminex Bio-Plex technology. The Bio-Plex suspension array component ofthe core has been in operation in the Evans laboratory for over 10 years and consists of a Luminex Bio-Plex workstation that will facilitate proteomic profiling of cytokines and chemokines in sufficiently small sample volumes compatible with plasma or culture fluids in a high throughput format.
Specific Aim 3 : To provide immunological support for investigators of the PPG. The Immunology Core will generate antisera against desired antigens and prepare a wide variety of immunological reagents to be used by the other Projects as requested, including primary or secondary antibodies to be used in immunoassays or Western blots or FACS analysis.
Specific Aim 4 : To provide quantitative and qualitative analysis of lipid and lipoprotein levels and provision of lipoprotein fractions. The core will provide measures of total cholesterol and triglycerides and will analyze FPLC profiles of murine plasma for these analytes and provide human LDL and/or other lipoproteins as needed

Public Health Relevance

The purpose of this core is to provide analytical expertise to the PPG investigators by making available specialized resources that will greatly facilitate the conduct of research. As specific questions are developed, targeted use of techniques available in this core can be developed.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
2P01HL088093-06A1
Application #
8703258
Study Section
Heart, Lung, and Blood Program Project Review Committee (HLBP)
Project Start
Project End
Budget Start
2014-05-01
Budget End
2015-04-30
Support Year
6
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of California San Diego
Department
Type
DUNS #
City
La Jolla
State
CA
Country
United States
Zip Code
92093
van der Valk, Fleur M; Bekkering, Siroon; Kroon, Jeffrey et al. (2016) Oxidized Phospholipids on Lipoprotein(a) Elicit Arterial Wall Inflammation and an Inflammatory Monocyte Response in Humans. Circulation 134:611-24
Zhao, Xuan; Hirota, Tsuyoshi; Han, Xuemei et al. (2016) Circadian Amplitude Regulation via FBXW7-Targeted REV-ERBα Degradation. Cell 165:1644-57
Wang, Junjian; Zou, June X; Xue, Xiaoqian et al. (2016) ROR-γ drives androgen receptor expression and represents a therapeutic target in castration-resistant prostate cancer. Nat Med 22:488-96
Verbeek, Rutger; Boekholdt, S Matthijs; Stoekenbroek, Robert M et al. (2016) Population and assay thresholds for the predictive value of lipoprotein (a) for coronary artery disease: the EPIC-Norfolk Prospective Population Study. J Lipid Res 57:697-705
Yang, Xiaohong; Lee, Sang-Rok; Choi, Yun-Seok et al. (2016) Reduction in lipoprotein-associated apoC-III levels following volanesorsen therapy: phase 2 randomized trial results. J Lipid Res 57:706-13
Gordts, Philip L S M; Nock, Ryan; Son, Ni-Huiping et al. (2016) ApoC-III inhibits clearance of triglyceride-rich lipoproteins through LDL family receptors. J Clin Invest 126:2855-66
Wall, Christopher E; Yu, Ruth T; Atkins, Anne R et al. (2016) Nuclear receptors and AMPK: can exercise mimetics cure diabetes? J Mol Endocrinol 57:R49-58
Liu, Weilin; Struik, Dicky; Nies, Vera J M et al. (2016) Effective treatment of steatosis and steatohepatitis by fibroblast growth factor 1 in mouse models of nonalcoholic fatty liver disease. Proc Natl Acad Sci U S A 113:2288-93
Ley, Klaus (2016) 2015 Russell Ross Memorial Lecture in Vascular Biology: Protective Autoimmunity in Atherosclerosis. Arterioscler Thromb Vasc Biol 36:429-38
Miller, Yury I; Shyy, John Y-J (2016) Context-Dependent Role of Oxidized Lipids and Lipoproteins in Inflammation. Trends Endocrinol Metab :

Showing the most recent 10 out of 113 publications