This proposal requests support to continue development of a Center of Biomedical Research Excellence in Protein Structure and Function (COBRE-PSF) at the University of Kansas. Our Center was established in October 2002, and includes participants from three additional Kansas Universities. After five years all of our initial goals have been met and, in most cases, surpassed by a wide margin. We have graduated nine junior investigators to independent R01 support, recruited and supported a total of 15 additional junior investigators through replacement and pilot project grants, held six scientific and three grant-writing workshops, and inspired multiple research collaborations. We also remodeled over 6000 sq. feet of space, built a new 17,500 sq. foot Structural Biology Center, inaugurated a new Core dedicated to Bio-NMR, and enacted plans to move COBRE-PSF toward a self-sustaining research center. Our investigators have published over 226 peer-reviewed manuscripts and have received 69 research grants totaling more than $22 M. For the renewal period, our overall Aims for the Center are: 1. To recruit, mentor, and accelerate the career development of outstanding junior scientists; 2. To enhance the capabilities of our existing Protein Production Core Laboratory (Core B); 3. To enhance the capabilities of our existing Protein Structure Laboratory (Core C); 4. To build a Bio-NMR Core Laboratory dedicated to Protein NMR studies (Core D); 5. To accelerate the professional and scientific growth of all Center participants through a combination of research meetings, seminars, grant application writing workshops, and scientific workshops. Our long-term goal is to attain a critical mass of successful, independent but collaborative investigators, focused on protein structure and function in health and disease, through which COBRE-PSF will become a self-sustaining enterprise into the future. Lay statement: Great advances in genomics paved the way for identifying the cellular proteins that are ultimately responsible for normal cell functioning and for the dysfunction underlying disease states. Development of therapeutic interventions begins with understanding the proteins involved in diseases, and this requires scientists with outstanding expertise in the study of cellular proteins.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
8P20GM103420-10
Application #
8258627
Study Section
National Center for Research Resources Initial Review Group (RIRG)
Program Officer
Canto, Maria Teresa
Project Start
2002-09-30
Project End
2014-03-31
Budget Start
2012-04-01
Budget End
2014-03-31
Support Year
10
Fiscal Year
2012
Total Cost
$1,994,159
Indirect Cost
$574,261
Name
University of Kansas Lawrence
Department
Pharmacology
Type
Schools of Pharmacy
DUNS #
076248616
City
Lawrence
State
KS
Country
United States
Zip Code
66045
Wahome, Newton; Sully, Erin; Singer, Christopher et al. (2016) Novel Ricin Subunit Antigens With Enhanced Capacity to Elicit Toxin-Neutralizing Antibody Responses in Mice. J Pharm Sci 105:1603-13
Estrada, D Fernando; Laurence, Jennifer S; Scott, Emily E (2016) Cytochrome P450 17A1 Interactions with the FMN Domain of Its Reductase as Characterized by NMR. J Biol Chem 291:3990-4003
Gowthaman, Ragul; Miller, Sven A; Rogers, Steven et al. (2016) DARC: Mapping Surface Topography by Ray-Casting for Effective Virtual Screening at Protein Interaction Sites. J Med Chem 59:4152-70
Budiardjo, S Jimmy; Licknack, Timothy J; Cory, Michael B et al. (2016) Full and Partial Agonism of a Designed Enzyme Switch. ACS Synth Biol 5:1475-1484
O'Neil, Pierce; Lovell, Scott; Mehzabeen, Nurjahan et al. (2016) Crystal structure of histone-like protein from Streptococcus mutans refined to 1.9 Å resolution. Acta Crystallogr F Struct Biol Commun 72:257-62
Meekins, David A; Zhang, Xin; Battaile, Kevin P et al. (2016) 1.45 Å resolution structure of SRPN18 from the malaria vector Anopheles gambiae. Acta Crystallogr F Struct Biol Commun 72:853-862
Damalanka, Vishnu C; Kim, Yunjeong; Alliston, Kevin R et al. (2016) Oxadiazole-Based Cell Permeable Macrocyclic Transition State Inhibitors of Norovirus 3CL Protease. J Med Chem 59:1899-913
Yadav, Rahul; Petrunak, Elyse M; Estrada, D Fernando et al. (2016) Structural insights into the function of steroidogenic cytochrome P450 17A1. Mol Cell Endocrinol :
Gurung, Ritu; Yadav, Rahul; Brungardt, Joseph G et al. (2016) Actin polymerization is stimulated by actin cross-linking protein palladin. Biochem J 473:383-96
Wani, Prashant S; Rowland, Michael A; Ondracek, Alex et al. (2015) Maturation of the proteasome core particle induces an affinity switch that controls regulatory particle association. Nat Commun 6:6384

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