The broad and long term goal of the Animal Models Core is provide consistent care, experimental procedures, and husbandry are important to the success ofthe projects. Each promising investigator has limited animal experimental experience, but needs to verify in vitro findings in vivo. Therefore, the Animal Model Core (AMC) will ensure project experimental consistency, compliance with federal animal welfare regulations, and effective training of project leaders and personnel in animal experimentation. Dr. Hinsdale will serve as the AMC director, and along with technical staff will provide basic services (e.g. Colony management and husbandry, biosafety containment and housing of animals, infection of animals and harvesting of samples for analyses, and pulmonary function tests). The current experimental animal models will be mice. The four projects that are proposed require mice to further verify in vivo findings arising from the proposed in vitro experiments. In project 1, using some remarkable viral strains, BALB/c mice will be used to determine the role of the human Respiratory Syncytial Virus (RSV) Matrix protein in viral infection. In project 2, influenza infected mice will be used to validate important host factors in an innovative in vitro respiratory model. In project 3, using a novel mouse model of acute lung injury, challenged mice will be used to mechanistically explore the exciting and potential therapeutic role of a unique protein on acute bacterial lung injury. In project 4, mice will be used to determine mechanistically the pathogenic role that specific chemokine and cytokine mediators have in acute lung injury due to influenza and to determine the pioneering therapeutic combinations for ameliorating this acute lung injury. In all, the up-and-coming young investigators of this application will be breaking new ground in pulmonary disease and gaining important animal model training to prepare them for promising independent research careers.

Public Health Relevance

The AMC as part of the proposed COBRE Center will provide a critical resource for the proposed studies into respiratory viral and bacterial diseases and to the discovery of mechanisms of lung injury and into therapeutics for infections of the lung. Furthermore, the AMC will be very crucial to the training of young investigators for independent research utilizing animal models of disease.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
5P20GM103648-02
Application #
8686889
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
2014-07-01
Budget End
2015-06-30
Support Year
2
Fiscal Year
2014
Total Cost
Indirect Cost
Name
Oklahoma State University Stillwater
Department
Type
DUNS #
City
Stillwater
State
OK
Country
United States
Zip Code
74078
Zhu, Liqian; Thunuguntla, Prasanth; Liu, Yilin et al. (2017) The ?-catenin signaling pathway stimulates bovine herpesvirus 1 productive infection. Virology 500:91-95
Feng, Yu; Zhao, Jianan; Chen, Xiaole et al. (2017) An In Silico Subject-Variability Study of Upper Airway Morphological Influence on the Airflow Regime in a Tracheobronchial Tree. Bioengineering (Basel) 4:
Eberle, R; Maxwell, L K; Nicholson, S et al. (2017) Genome sequence variation among isolates of monkey B virus (Macacine alphaherpesvirus 1) from captive macaques. Virology 508:26-35
Wang, Xiaoqiu; Wu, Wenxin; Zhang, Wei et al. (2017) RIG-I overexpression decreases mortality of cigarette smoke exposed mice during influenza A virus infection. Respir Res 18:166
Premaratne, Gayan; Al Mubarak, Zainab H; Senavirathna, Lakmini et al. (2017) Measuring Ultra-low Levels of Nucleotide Biomarkers Using Quartz Crystal Microbalance and SPR Microarray Imaging Methods: A Comparative Analysis. Sens Actuators B Chem 253:368-375
Fu, Bishi; Zhao, Mengmeng; Wang, Lingyan et al. (2017) RNAi Screen and Proteomics Reveal NXF1 as a Novel Regulator of IRF5 Signaling. Sci Rep 7:2683
Wu, Lei; Guo, Xin; Hartson, Steven D et al. (2017) Lack of ?, ?-carotene-9', 10'-oxygenase 2 leads to hepatic mitochondrial dysfunction and cellular oxidative stress in mice. Mol Nutr Food Res 61:
Fu, Bishi; Wang, Lingyan; Li, Shitao et al. (2017) ZMPSTE24 defends against influenza and other pathogenic viruses. J Exp Med 214:919-929
Zeng, Xiangming; Huang, Chaoqun; Senavirathna, Lakmini et al. (2017) miR-27b inhibits fibroblast activation via targeting TGF? signaling pathway. BMC Cell Biol 18:9
Zhu, Liqian; Thompson, Jesse; Ma, Fangrui et al. (2017) Effects of the synthetic corticosteroid dexamethasone on bovine herpesvirus 1 productive infection. Virology 505:71-79

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