This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Microbial evolution occurs on a time scale that is rapid relative to the human lifespan and can lead to changes in virulence, host and drug resistance. The long term goal of this project is to determine whether there are general principles that can be used to predict the molecular basis of short term adaptive evolution. The focus of the proposal is to develop a deeper understanding of the mechanistic basis of adaptive evolution using icosahedral bacteriophage as a model system.
The specific aims are 1) to determine the structural basis of protein adaptation using biochemical and biophysical principles;and 2) to determine the contribution of regulatory substitutions to adaptive evolution. To address the first aim, we will identify amino acid substitutions that increase or decrease temperature range in evolved phage and dissect the biophysical mechanisms underlying these adaptations. We will use this information to build a predictive model of thermal adaptation and test this model by predicting the relative temperature range of phages taken from the environment, and by constructing predicted thermal mutants in a related phage. To address the second aim, we will use gene expression and phenotypic assays to determine which experimentally observed changes in regulatory regions exert their adaptive effects by altering gene expression. We will also determine the magnitude of the changes and the context in which they are adaptive and identify selective conditions that promote changes in gene regulation. Successful achievement of these aims will enhance our ability to predict the probable pathways of adaptive evolution.
|Marx, Hannah E; Dentant, Cédric; Renaud, Julien et al. (2017) Riders in the sky (islands): using a mega-phylogenetic approach to understand plant species distribution and coexistence at the altitudinal limits of angiosperm plant life. J Biogeogr 44:2618-2630|
|Sarver, Brice A J; Demboski, John R; Good, Jeffrey M et al. (2016) Comparative Phylogenomic Assessment of Mitochondrial Introgression among Several Species of Chipmunks (TAMIAS). Genome Biol Evol :|
|Stockmann, Chris; Ampofo, Krow; Pavia, Andrew T et al. (2016) Clinical and Epidemiological Evidence of the Red Queen Hypothesis in Pneumococcal Serotype Dynamics. Clin Infect Dis 63:619-626|
|Loftie-Eaton, Wesley; Yano, Hirokazu; Burleigh, Stephen et al. (2016) Evolutionary Paths That Expand Plasmid Host-Range: Implications for Spread of Antibiotic Resistance. Mol Biol Evol 33:885-97|
|Yano, Hirokazu; Wegrzyn, Katarznya; Loftie-Eaton, Wesley et al. (2016) Evolved plasmid-host interactions reduce plasmid interference cost. Mol Microbiol 101:743-56|
|Chernikova, Diana A; Koestler, Devin C; Hoen, Anne Gatewood et al. (2016) Fetal exposures and perinatal influences on the stool microbiota of premature infants. J Matern Fetal Neonatal Med 29:99-105|
|Uribe-Convers, Simon; Settles, Matthew L; Tank, David C (2016) A Phylogenomic Approach Based on PCR Target Enrichment and High Throughput Sequencing: Resolving the Diversity within the South American Species of Bartsia L. (Orobanchaceae). PLoS One 11:e0148203|
|Garud, Nandita R; Messer, Philipp W; Buzbas, Erkan O et al. (2015) Recent selective sweeps in North American Drosophila melanogaster show signatures of soft sweeps. PLoS Genet 11:e1005004|
|Metzger, Genevieve; Espindola, Anahi; Waits, Lisette P et al. (2015) Genetic Structure across Broad Spatial and Temporal Scales: Rocky Mountain Tailed Frogs (Ascaphus montanus; Anura: Ascaphidae) in the Inland Temperate Rainforest. J Hered 106:700-10|
|Loftie-Eaton, Wesley; Suzuki, Haruo; Bashford, Kelsie et al. (2015) Draft Genome Sequence of Pseudomonas sp. nov. H2. Genome Announc 3:|
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