9.1.3 EXPERIMENTAL MOUSE SHARED SERVICE The Experimental Mouse Shared Service (EMSS) is a one-stop shared service for all your in vivo mouse experimentation needs. The purpose of the EMSS is to provide investigators at the Arizona Cancer Center (AZCC) with experimental mouse models and with a wide range of mouse experimentation techniques. The EMSS has combined the previous Genetically Engineered Mouse "Developmental" Shared Service (GEMSS) and the previous EMSS. Therefore, the EMSS begins with the design and construction of gene targeting and transgenic mouse vectors, gene targeting and ES cell screening for targeted Clones, production of genetargeted mice, production of transgenic mice, screening for transgenic and knockout founders, embryo rederivation for pathogen cleanup and cryopreservation of mouse strains through to complete experimentation services in all aspects of xenograft and GEM models. The specific objectives of the EMSS include creation of models, maintenance of shared and investigator colonies, assistance in the design of in vivo experiments, and performance of mouse experiments by personnel with expertise in mouse experimental protocols. Hence, the EMSS provides consistency and quality of procedures within and between mouse experiments that ensures accuracy in experimental results, confidence in interpretation of results, and an inner-consistency between mouse experiments which is essential for intra-lab and inter-lab comparative analyses. Due to the fact that the mouse is a model for all translational cancer work for both NIH-type funding as well as progression to clinical trials, the EMSS is an absolutely essential shared service. The benefits of the EMSS are to provide AZCC investigators with a cost-effective mechanism for the creation of different mouse models and for completing in vivo mouse experiments utilizing highly trained and experienced technicians. The EMSS continues to expand its services in a very cost-effective and efficient manner.

Public Health Relevance

Provides a continuum of services for AZCC investigators that ranges from experimental design, to GEM production, to mouse experimentation, thereby enabling the AZCC investigator to generate and utilize mouse models of human cancer to their fullest potential without the investigator having to be an expert on mouse genetic engineering or mouse experimentation.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA023074-35
Application #
8540933
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2013-07-01
Budget End
2014-06-30
Support Year
35
Fiscal Year
2013
Total Cost
$169,584
Indirect Cost
$58,006
Name
University of Arizona
Department
Type
DUNS #
806345617
City
Tucson
State
AZ
Country
United States
Zip Code
85721
Dickinson, Sally E; Janda, Jaroslav; Criswell, Jane et al. (2016) Inhibition of Akt Enhances the Chemopreventive Effects of Topical Rapamycin in Mouse Skin. Cancer Prev Res (Phila) 9:215-24
Schissler, A Grant; Li, Qike; Chen, James L et al. (2016) Analysis of aggregated cell-cell statistical distances within pathways unveils therapeutic-resistance mechanisms in circulating tumor cells. Bioinformatics 32:i80-i89
Banerjee, Bhaskar; Shaheen, Nicholas J; Martinez, Jessica A et al. (2016) Clinical Study of Ursodeoxycholic Acid in Barrett's Esophagus Patients. Cancer Prev Res (Phila) 9:528-33
Pulko, Vesna; Davies, John S; Martinez, Carmine et al. (2016) Human memory T cells with a naive phenotype accumulate with aging and respond to persistent viruses. Nat Immunol 17:966-75
Warfel, Noel A; Sainz, Alva G; Song, Jin H et al. (2016) PIM Kinase Inhibitors Kill Hypoxic Tumor Cells by Reducing Nrf2 Signaling and Increasing Reactive Oxygen Species. Mol Cancer Ther 15:1637-47
Thomson, Cynthia A; Ho, Emily; Strom, Meghan B (2016) Chemopreventive properties of 3,3'-diindolylmethane in breast cancer: evidence from experimental and human studies. Nutr Rev 74:432-43
Tsikitis, Vassiliki L; Potter, Amiee; Mori, Motomi et al. (2016) MicroRNA Signatures of Colonic Polyps on Screening and Histology. Cancer Prev Res (Phila) 9:942-949
Hanke, Neale T; Garland, Linda L; Baker, Amanda F (2016) Carfilzomib combined with suberanilohydroxamic acid (SAHA) synergistically promotes endoplasmic reticulum stress in non-small cell lung cancer cell lines. J Cancer Res Clin Oncol 142:549-60
Kohler, Lindsay N; Garcia, David O; Harris, Robin B et al. (2016) Adherence to Diet and Physical Activity Cancer Prevention Guidelines and Cancer Outcomes: A Systematic Review. Cancer Epidemiol Biomarkers Prev 25:1018-28
Landowski, Terry H; Guntle, Gerald P; Zhao, Dezheng et al. (2016) Magnetic Resonance Imaging Identifies Differential Response to Pro-Oxidant Chemotherapy in a Xenograft Model. Transl Oncol 9:228-35

Showing the most recent 10 out of 1161 publications