? Microscopy Imaging and Cellular Physiology Core The Microscopy Imaging and Cellular Physiology (MICP) Core provides members of the Michigan Diabetes Research Center access to state of the art microscopy imaging, in situ hybridization, and electrophysiologic (including optogenetics) analysis, along with expert analysis and support. For over 20 years, the MICPC has provided researchers use of confocal and wide-field microscopes to allow a wide array of cellular and tissue imaging techniques including; imaging of fixed tissues and cells, quantification, co-localization, and live cell imaging including FRET and FRAP experiments. We have now added in situ hybridization analysis with fluorescent or radioisotope or chromogenic substrates for localization and/or quantification of mRNA. The core also has also developed an electrophysiology laboratory that enables the analysis of electrical and ionic changes in neurons, islets, or other relevant tissues, including optogentic activation of these tissues. Core personnel provide extensive expertise in imaging, in situ hybridization and electrophysiology, enabling MDRC investigators to rapidly develop novel experimental ideas and obtain high quality results with expert analysis. Core personnel provide structured service, maintenance and expertise in imaging and cell physiology and related experiments to support diabetes research at Michigan.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK020572-42
Application #
9657011
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2018-12-01
Budget End
2019-11-30
Support Year
42
Fiscal Year
2019
Total Cost
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Type
DUNS #
073133571
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
Kimball, Andrew; Schaller, Matthew; Joshi, Amrita et al. (2018) Ly6CHi Blood Monocyte/Macrophage Drive Chronic Inflammation and Impair Wound Healing in Diabetes Mellitus. Arterioscler Thromb Vasc Biol 38:1102-1114
Lee, Jin-Sook; Caruso, Joseph A; Hubbs, Garrett et al. (2018) Molecular architecture of mouse and human pancreatic zymogen granules: protein components and their copy numbers. Biophys Rep 4:94-103
Yue, Yang; Blasius, T Lynne; Zhang, Stephanie et al. (2018) Altered chemomechanical coupling causes impaired motility of the kinesin-4 motors KIF27 and KIF7. J Cell Biol 217:1319-1334
Ammari, Zaid; Pak, Stella C; Ruzieh, Mohammed et al. (2018) Posttransplant Tacrolimus-Induced Diabetic Ketoacidosis: Review of the Literature. Case Rep Endocrinol 2018:4606491
Brown, Callie L; Perrin, Eliana M; Peterson, Karen E et al. (2018) Association of Picky Eating With Weight Status and Dietary Quality Among Low-Income Preschoolers. Acad Pediatr 18:334-341
Rodriquez, Erik J; Livaudais-Toman, Jennifer; Gregorich, Steven E et al. (2018) Relationships between allostatic load, unhealthy behaviors, and depressive disorder in U.S. adults, 2005-2012 NHANES. Prev Med 110:9-15
Morran, Michael P; Al-Dieri, Ali G; Nestor-Kalinoski, Andrea L et al. (2018) Insulin receptor based lymphocyte trafficking in the progression of type 1 diabetes. J Biol Methods 5:
Jiang, Youde; Liu, Li; Steinle, Jena J (2018) miRNA15a regulates insulin signal transduction in the retinal vasculature. Cell Signal 44:28-32
Montrose, Luke; Padmanabhan, Vasantha; Goodrich, Jaclyn M et al. (2018) Maternal levels of endocrine disrupting chemicals in the first trimester of pregnancy are associated with infant cord blood DNA methylation. Epigenetics 13:301-309
Afshinnia, Farsad; Rajendiran, Thekkelnaycke M; Wernisch, Stefanie et al. (2018) Lipidomics and Biomarker Discovery in Kidney Disease. Semin Nephrol 38:127-141

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