Module 3. Molecular Biology and Genotyping (MBG) Dr. Palczewski, Director, Ganna Yakubenko, MS, Manager Overview and Resources: The Molecular Biology Module has been in effect since the flrst funding period of the Case VSRC P30 grant in 1997. In the most recent funding period, the Module has worked especially closely with the Specialized Animal Resource Module to genotype established mouse strains and to characterize newly generated strains using the Cre-Lox recombinase system. Although genotyping is the predominant function of this Module, it also comprises a BioRad Versa Doc Imaging Systemc and a new Eppendorf Realplex2 Master Cycler qPCR machine in addition to the Applied Bioscience qPCR thermocycler, all of which are extensively used by VSRC investigators. Overall, we anticipate that the Module will continue to be very heavily utilized. The MBG Module is located in the centralized VSRC facilities in the Institute of Pathology, and provides technical expertise, access to equipment and techniques for PCR analysis and genotyping, quantitative PCR, and imaging of agarose and acrylamide gels.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
5P30EY011373-17
Application #
8550067
Study Section
Special Emphasis Panel (ZEY1-VSN)
Project Start
Project End
Budget Start
2013-09-01
Budget End
2014-08-31
Support Year
17
Fiscal Year
2013
Total Cost
$144,632
Indirect Cost
$52,510
Name
Case Western Reserve University
Department
Type
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106
Wang, Hua; Linetsky, Mikhail; Guo, Junhong et al. (2016) Metabolism of 4-Hydroxy-7-oxo-5-heptenoic Acid (HOHA) Lactone by Retinal Pigmented Epithelial Cells. Chem Res Toxicol 29:1198-210
Wensel, Theodore G; Zhang, Zhixian; Anastassov, Ivan A et al. (2016) Structural and molecular bases of rod photoreceptor morphogenesis and disease. Prog Retin Eye Res 55:32-51
Palczewski, Grzegorz; Widjaja-Adhi, M Airanthi K; Amengual, Jaume et al. (2016) Genetic dissection in a mouse model reveals interactions between carotenoids and lipid metabolism. J Lipid Res 57:1684-95
Chen, Yu; Perusek, Lindsay; Maeda, Akiko (2016) Autophagy in light-induced retinal damage. Exp Eye Res 144:64-72
Sahu, Bhubanananda; Maeda, Akiko (2016) Retinol Dehydrogenases Regulate Vitamin A Metabolism for Visual Function. Nutrients 8:
Whitson, Jeremy A; Sell, David R; Goodman, Michael C et al. (2016) Evidence of Dual Mechanisms of Glutathione Uptake in the Rodent Lens: A Novel Role for Vitreous Humor in Lens Glutathione Homeostasis. Invest Ophthalmol Vis Sci 57:3914-25
Fan, Xingjun; Monnier, Vincent M; Whitson, Jeremy (2016) Lens glutathione homeostasis: Discrepancies and gaps in knowledge standing in the way of novel therapeutic approaches. Exp Eye Res :
Saadane, Aicha; Mast, Natalia; Dao, Tung et al. (2016) Retinal Hypercholesterolemia Triggers Cholesterol Accumulation and Esterification in Photoreceptor Cells. J Biol Chem 291:20427-39
Taylor, Patricia R; Roy, Sanhita; Meszaros, Evan C et al. (2016) JAK/STAT regulation of Aspergillus fumigatus corneal infections and IL-6/23-stimulated neutrophil, IL-17, elastase, and MMP9 activity. J Leukoc Biol 100:213-22
Mishra, Ashish K; Gragg, Megan; Stoneman, Michael R et al. (2016) Quaternary structures of opsin in live cells revealed by FRET spectrometry. Biochem J 473:3819-3836

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