Abstract

Module 3. Molecular Biology and Genotyping (MBG) Dr. Palczewski, Director, Ganna Yakubenko, MS, Manager Overview and Resources: The Molecular Biology Module has been in effect since the flrst funding period of the Case VSRC P30 grant in 1997. In the most recent funding period, the Module has worked especially closely with the Specialized Animal Resource Module to genotype established mouse strains and to characterize newly generated strains using the Cre-Lox recombinase system. Although genotyping is the predominant function of this Module, it also comprises a BioRad Versa Doc Imaging Systemc and a new Eppendorf Realplex2 Master Cycler qPCR machine in addition to the Applied Bioscience qPCR thermocycler, all of which are extensively used by VSRC investigators. Overall, we anticipate that the Module will continue to be very heavily utilized. The MBG Module is located in the centralized VSRC facilities in the Institute of Pathology, and provides technical expertise, access to equipment and techniques for PCR analysis and genotyping, quantitative PCR, and imaging of agarose and acrylamide gels.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
5P30EY011373-17
Application #
8550067
Study Section
Special Emphasis Panel (ZEY1-VSN)
Project Start
Project End
Budget Start
2013-09-01
Budget End
2014-08-31
Support Year
17
Fiscal Year
2013
Total Cost
$144,632
Indirect Cost
$52,510

  Institution

Name
Case Western Reserve University
Department
Type
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106

  Publications

Malemud, Charles J (2018) The role of the JAK/STAT signal pathway in rheumatoid arthritis. Ther Adv Musculoskelet Dis 10:117-127
Clark, Heather L; Minns, Martin S; Sun, Yan et al. (2018) Atovaquone Impairs Growth of Aspergillus and Fusarium Keratitis Isolates by Modulating Mitochondrial Function and Zinc Homeostasis. Invest Ophthalmol Vis Sci 59:1589-1598
Parmar, Tanu; Parmar, Vipul M; Perusek, Lindsay et al. (2018) Lipocalin 2 Plays an Important Role in Regulating Inflammation in Retinal Degeneration. J Immunol 200:3128-3141
Widjaja-Adhi, Made Airanthi K; Ramkumar, Srinivasagan; von Lintig, Johannes (2018) Protective role of carotenoids in the visual cycle. FASEB J :fj201800467R
Chen, Yuanyuan; Chen, Yu; Jastrzebska, Beata et al. (2018) A novel small molecule chaperone of rod opsin and its potential therapy for retinal degeneration. Nat Commun 9:1976
Mast, Natalia; Bederman, Ilya R; Pikuleva, Irina A (2018) Retinal Cholesterol Content Is Reduced in Simvastatin-Treated Mice Due to Inhibited Local Biosynthesis Albeit Increased Uptake of Serum Cholesterol. Drug Metab Dispos 46:1528-1537
Hughes, Taylor E T; Lodowski, David T; Huynh, Kevin W et al. (2018) Structural basis of TRPV5 channel inhibition by econazole revealed by cryo-EM. Nat Struct Mol Biol 25:53-60
Hughes, Taylor E T; Pumroy, Ruth A; Yazici, Aysenur Torun et al. (2018) Structural insights on TRPV5 gating by endogenous modulators. Nat Commun 9:4198
Gulati, Sahil; Jin, Hui; Masuho, Ikuo et al. (2018) Targeting G protein-coupled receptor signaling at the G protein level with a selective nanobody inhibitor. Nat Commun 9:1996
Senapati, Subhadip; Gragg, Megan; Samuels, Ivy S et al. (2018) Effect of dietary docosahexaenoic acid on rhodopsin content and packing in photoreceptor cell membranes. Biochim Biophys Acta Biomembr 1860:1403-1413

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