Abstract

The toxicity of free radicals produced in cellular oxidative reactions is well known. In eye tissues, the frequent exposure to light poses an additional source of free radical. Peroxidase is the major enzyme system i the retinal pigment epithelium to prevent accumulation of H2O2. The detail d biochemical nature of this enzyme in the retinal pigment epithelium and its identity of the peroxidase of other tissues is not known. The present specific aim is to purify the major peroxidase from the retinal pigment epithelium, compare its properties with that of erythrocytes, granulocytes, and to produce a specific antibody for the purified peroxidase. The antibo y will be used to visualize its location in the eye tissues.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Research Project (R01)
Project #
5R01EY008213-03
Application #
3265422
Study Section
Visual Sciences A Study Section (VISA)
Project Start
1989-09-01
Project End
1993-08-31
Budget Start
1991-09-01
Budget End
1993-08-31
Support Year
3
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
University of Texas Health Science Center San Antonio
Department
Type
Schools of Medicine
DUNS #
800772162
City
San Antonio
State
TX
Country
United States
Zip Code
78229

  Publications

White, Jacqueline K; Gerdin, Anna-Karin; Karp, Natasha A et al. (2013) Genome-wide generation and systematic phenotyping of knockout mice reveals new roles for many genes. Cell 154:452-64
Wang, L; Lam, K W; Lam, T T et al. (1994) Comparison of peroxidases in the retina, choroid and red blood cells. Ophthalmic Res 26:219-25
Wang, L; Lam, T T; Lam, K W et al. (1994) Correlation of phospholipid hydroperoxide glutathione peroxidase activity to the sensitivity of rat retinas to photic injury. Ophthalmic Res 26:60-4
Lam, K W; Zwaan, J; Garcia, A et al. (1993) Detection of ascorbic acid in the eye of the early chicken embryo by silver staining. Exp Eye Res 56:601-4
Lam, K W; Yu, H S; Glickman, R D et al. (1993) Sodium-dependent ascorbic and dehydroascorbic acid uptake by SV-40-transformed retinal pigment epithelial cells. Ophthalmic Res 25:100-7
Glickman, R D; Sowell, R; Lam, K W (1993) Kinetic properties of light-dependent ascorbic acid oxidation by melanin. Free Radic Biol Med 15:453-7
Lam, K W; Wang, L; Hong, B S et al. (1993) Purification of phospholipid hydroperoxide glutathione peroxidase from bovine retina. Curr Eye Res 12:9-15
Glickman, R D; Lam, K W (1992) Oxidation of ascorbic acid as an indicator of photooxidative stress in the eye. Photochem Photobiol 55:191-6
Zwaan, J; Lam, K W (1992) Comparison of ascorbic acid levels in the eye and remainder of the chicken embryo during development. Exp Eye Res 54:411-3
Yu, H S; Pastor, S A; Lam, K W et al. (1990) Ascorbate-enhanced copper toxicity on bovine corneal endothelial cells in vitro. Curr Eye Res 9:177-82