The fundamental physical properties of the outer tunic of the eye determine the structural characteristics of the ocular globe and may be altered in several disease states including axial elongation in myopia, pathological deformation in keratoconus, and iatrogenic keratoectasia following corneal refractive surgery. These biomechanical tissue characteristics not only influence our clinical interpretation of diagnostic tests, e.g. measurement of intraocular pressure, but have been implicated as important factors in the development of glaucoma and other diseases. Currently, there is no reliable method to perform measurements of corneal structural properties in vivo. Here we will develop a novel method for the topographical assessment of corneal elastic properties that could potentially be used for routine clinical diagnosis and monitoring of treatment. This method will take advantage of localized pulsed-air stimulation to generate microscopic pressure waves within the cornea and use phase-sensitive swept-source optical coherence tomography to detect and analyze the resultant pressure wave propagation within the cornea to reconstruct volumetric biomechanical properties of this tissue. Our long-term objectives are to use the coordinated talents of this research team to produce novel elasticity imaging instrumentation/methods that can extend our current understanding of the basic principles of tissue biomechanics and apply this knowledge to clinically relevant problems in ocular disease.

Public Health Relevance

This proposal will develop novel technology and methods for noninvasive reconstruction of biomechanical properties of the cornea. Development of such a technique would significantly advance our understanding of the corneal disorders, allow developing novel clinical therapies and interventions, and improve outcome of current surgical interventions including corneal refractive surgery.

National Institute of Health (NIH)
National Eye Institute (NEI)
Research Project (R01)
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Special Emphasis Panel (ZRG1-NT-L (09))
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Wiggs, Cheri
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University of Houston
Engineering (All Types)
Schools of Engineering
United States
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Singh, Manmohan; Wang, Shang; Yee, Richard W et al. (2016) Optical coherence tomography as a tool for real-time visual feedback and biomechanical assessment of dermal filler injections: preliminary results in a pig skin model. Exp Dermatol 25:475-6
Wu, Chen; Singh, Manmohan; Han, Zhaolong et al. (2016) Lorentz force optical coherence elastography. J Biomed Opt 21:90502
Singh, Manmohan; Li, Jiasong; Vantipalli, Srilatha et al. (2016) Noncontact Elastic Wave Imaging Optical Coherence Elastography for Evaluating Changes in Corneal Elasticity Due to Crosslinking. IEEE J Sel Top Quantum Electron 22:
Singh, Manmohan; Li, Jiasong; Han, Zhaolong et al. (2016) Evaluating the Effects of Riboflavin/UV-A and Rose-Bengal/Green Light Cross-Linking of the Rabbit Cornea by Noncontact Optical Coherence Elastography. Invest Ophthalmol Vis Sci 57:OCT112-20
Du, Yong; Liu, Chih-Hao; Lei, Ling et al. (2016) Rapid, noninvasive quantitation of skin disease in systemic sclerosis using optical coherence elastography. J Biomed Opt 21:46002
Wang, Shang; Singh, Manmohan; Lopez 3rd, Andrew L et al. (2015) Direct four-dimensional structural and functional imaging of cardiovascular dynamics in mouse embryos with 1.5 MHz optical coherence tomography. Opt Lett 40:4791-4
Wu, Chen; Han, Zhaolong; Wang, Shang et al. (2015) Assessing age-related changes in the biomechanical properties of rabbit lens using a coaligned ultrasound and optical coherence elastography system. Invest Ophthalmol Vis Sci 56:1292-300
Aglyamov, Salavat R; Wang, Shang; Karpiouk, Andrei B et al. (2015) The dynamic deformation of a layered viscoelastic medium under surface excitation. Phys Med Biol 60:4295-312
Singh, Manmohan; Wu, Chen; Liu, Chih-Hao et al. (2015) Phase-sensitive optical coherence elastography at 1.5 million A-Lines per second. Opt Lett 40:2588-91
Han, Zhaolong; Li, Jiasong; Singh, Manmohan et al. (2015) Quantitative methods for reconstructing tissue biomechanical properties in optical coherence elastography: a comparison study. Phys Med Biol 60:3531-47

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