The overall goal is to establish a communal genomic resource that will facilitate and accelerate biomedical and biological research in the sea lamprey (Petromyzon marinus), a model for vertebrate evolution, disease processes and behavioral mechanisms. Lampreys are survivors from the jawless stage of vertebrate evolution. Studies using the sea lamprey as a model organism have helped understand evolution of jaws, fins, neural crest and adaptive immunity. In addition, the sea lamprey is a primary model for spinal cord injury and function, and recently has been used in neurodegenerative disease. Finally, radical changes in many organs during metamorphosis offer unique and efficient, but currently underutilized, models for understanding numerous liver diseases and autoimmune responses. However, genomic resources for the sea lamprey have been surprisingly poor for a species with such a crucial evolutionary niche and demonstrated utility in biomedical and development research. It was identified that comprehensive transcriptomes and high density microarrays, when shared among the community, can enhance the existing resources and enable an explosive growth in lamprey research. This proposed project will accomplish two objectives: (1) establish comprehensive transcriptomes across different tissues throughout the life history;and (2) develop a high density cDNA oligonucleotide microarray. When these two objectives are accomplished, the resulted resources will accelerate discoveries that may lead cure to spinal cord injury and neurodegenerative disease. These resources will help understand many other disease processes.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Resource-Related Research Projects (R24)
Project #
5R24GM083982-02
Application #
7741251
Study Section
Genomics, Computational Biology and Technology Study Section (GCAT)
Program Officer
Tompkins, Laurie
Project Start
2008-12-01
Project End
2011-11-30
Budget Start
2009-12-01
Budget End
2010-11-30
Support Year
2
Fiscal Year
2010
Total Cost
$378,550
Indirect Cost
Name
Michigan State University
Department
Zoology
Type
Schools of Earth Sciences/Natur
DUNS #
193247145
City
East Lansing
State
MI
Country
United States
Zip Code
48824
Chung-Davidson, Yuwen; Wang, Huiyong; Scott, Anne M et al. (2015) Pheromone 3kPZS evokes context-dependent serotonin sexual dimorphism in the brain of the sea lamprey (Petromyzon marinus). Integr Zool 10:91-101
Chung-Davidson, Yu-Wen; Yeh, Chu-Yin; Bussy, Ugo et al. (2015) Hsp90 and hepatobiliary transformation during sea lamprey metamorphosis. BMC Dev Biol 15:47
Wang, Huiyong; Yeh, Chu-Yin; Li, Ke et al. (2015) An UPLC-MS/MS method for quantitative profiling of bile acids in sea lamprey plasma and tissues. J Chromatogr B Analyt Technol Biomed Life Sci 980:72-8
Chung-Davidson, Yu-Wen; Priess, M Cody; Yeh, Chu-Yin et al. (2013) A thermogenic secondary sexual character in male sea lamprey. J Exp Biol 216:2702-12
Smith, Jeramiah J; Kuraku, Shigehiro; Holt, Carson et al. (2013) Sequencing of the sea lamprey (Petromyzon marinus) genome provides insights into vertebrate evolution. Nat Genet 45:415-21, 421e1-2
Chang, Steven; Chung-Davidson, Yu-Wen; Libants, Scot V et al. (2013) The sea lamprey has a primordial accessory olfactory system. BMC Evol Biol 13:172
Walaszczyk, Erin J; Johnson, Nicholas S; Steibel, Juan Pedro et al. (2013) Effects of sex pheromones and sexual maturation on locomotor activity in female sea lamprey (Petromyzon marinus). J Biol Rhythms 28:218-26
Chung-Davidson, Yu-Wen; Wang, Huiyong; Bryan, Mara B et al. (2013) An anti-steroidogenic inhibitory primer pheromone in male sea lamprey (Petromyzon marinus). Gen Comp Endocrinol 189:24-31
Brant, Cory O; Chung-Davidson, Yu-Wen; Li, Ke et al. (2013) Biosynthesis and release of pheromonal bile salts in mature male sea lamprey. BMC Biochem 14:30
Chung-Davidson, Yu-Wen; Wang, Huiyong; Siefkes, Michael J et al. (2013) Pheromonal bile acid 3-ketopetromyzonol sulfate primes the neuroendocrine system in sea lamprey. BMC Neurosci 14:11

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