This proposal seeks funds to establish a Center for Humanized Mice Development. This new facility at the University of Nebraska Medical Center (UNMC) will offer environmentally, genetically, and xenotransplantation- engineered mouse models for translational studies and drug discovery, which are the mainstream at UNMC. It is well known that often clinical studies are successful in animal models but fail at the human level. Also the focused use of micro-animals, mice in particular, makes applicability to humans problematic. In turn, we are developing a resource that will generate improved animal models to study human immunity, human-specific infections, vaccines, and human-specific drug interactions. The resources are expected to be efficiently utilized for speeding the translation of new therapeutics to patients. Our goals to improve existing strains of mice are as follows: modify mouse backgrounds for the studies of human-like adaptive immune responses to broader range of pathogens and evaluation of vaccine candidates, create strains of mice that are compatible with the function of human immune system based on human-like glycosilation patterns, modify mouse backgrounds for studies of human-like drug metabolism and drug interaction, study HIV-1-related co-infections, such as hepatitis, tuberculosis, and malaria, and lastly examine HIV-1-associated comorbidities, including end-organ diseases like HIV-1-associated neurocognitive disorders (HAND).
Nebraska Center for Humanized Mice Development will provide biomedical community with environmentally, genetically, and xenotransplantation-modified mice for the studies on human immunity, viral infections/co-infections and related comorbidities, drug interactions, and vaccines.
|Gurumurthy, Channabasavaiah B; Grati, M'hamed; Ohtsuka, Masato et al. (2016) CRISPR: a versatile tool for both forward and reverse genetics research. Hum Genet 135:971-6|
|Quadros, Rolen M; Poluektova, Larisa Y; Gurumurthy, Channabasavaiah B (2016) Simple and reliable genotyping protocol for mouse Prkdc(SCID) mutation. J Immunol Methods 431:60-2|
|AraÃnga, Mariluz; Su, Hang; Poluektova, Larisa Y et al. (2016) HIV-1 cellular and tissue replication patterns in infected humanized mice. Sci Rep 6:23513|
|Akkina, Ramesh; Allam, Atef; Balazs, Alejandro B et al. (2016) Improvements and Limitations of Humanized Mouse Models for HIV Research: NIH/NIAID "Meet the Experts" 2015 Workshop Summary. AIDS Res Hum Retroviruses 32:109-19|
|Li, Weizhe; Tong, Hsin-I; Gorantla, Santhi et al. (2016) Neuropharmacologic Approaches to Restore the Brain's Microenvironment. J Neuroimmune Pharmacol 11:484-94|
|Bade, Aditya N; Gorantla, Santhi; Dash, Prasanta K et al. (2016) Manganese-Enhanced Magnetic Resonance Imaging Reflects Brain Pathology During Progressive HIV-1 Infection of Humanized Mice. Mol Neurobiol 53:3286-97|
|Zhang, Jinjin; Garrison, Jered C; Poluektova, Larisa Y et al. (2015) Liver-targeted antiviral peptide nanocomplexes as potential anti-HCV therapeutics. Biomaterials 70:37-47|
|Gurumurthy, Channabasavaiah B; Joshi, Poonam S; Kurz, Scott G et al. (2015) Validation of simple sequence length polymorphism regions of commonly used mouse strains for marker assisted speed congenics screening. Int J Genomics 2015:735845|
|Knibbe-Hollinger, Jaclyn S; Fields, Natasha R; Chaudoin, Tammy R et al. (2015) Influence of age, irradiation and humanization on NSG mouse phenotypes. Biol Open 4:1243-52|
|Boska, Michael D; Dash, Prasanta K; Knibbe, Jaclyn et al. (2014) Associations between brain microstructures, metabolites, and cognitive deficits during chronic HIV-1 infection of humanized mice. Mol Neurodegener 9:58|
Showing the most recent 10 out of 12 publications