Flow Cytometry is the primary tool in most immunology laboratories for the detailed characterization of lymphocytes and lymphocyte function. The ability to resolve highly complex populations of cells, interrogate the cells one-by-one, make multiple measurements on each cell, and then separate the cells viably for further study make this technology unique and indispensable. Our laboratory has long been involved in pushing this technology forward;we have perhaps the most sophisticated flow cytometer in the world, currently capable of making 20 different measurements on each cell. This platform has proven invaluable in dissecting immune responses to vaccines and viruses. In addition, we are developing novel algorithms for the automated analysis of the highly complex data that results from these experiments.

Project Start
Project End
Budget Start
Budget End
Support Year
12
Fiscal Year
2013
Total Cost
$1,168,546
Indirect Cost
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State
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Fletez-Brant, Kipper; Å pidlen, Josef; Brinkman, Ryan R et al. (2016) flowClean: Automated identification and removal of fluorescence anomalies in flow cytometry data. Cytometry A 89:461-71
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Pilipow, Karolina; Roberto, Alessandra; Roederer, Mario et al. (2015) IL15 and T-cell Stemness in T-cell-Based Cancer Immunotherapy. Cancer Res 75:5187-93
Shen, Xiaoying; Duffy, Ryan; Howington, Robert et al. (2015) Vaccine-Induced Linear Epitope-Specific Antibodies to Simian Immunodeficiency Virus SIVmac239 Envelope Are Distinct from Those Induced to the Human Immunodeficiency Virus Type 1 Envelope in Nonhuman Primates. J Virol 89:8643-50

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