Flow Cytometry is the primary tool in most immunology laboratories for the detailed characterization of lymphocytes and lymphocyte function. The ability to resolve highly complex populations of cells, interrogate the cells one-by-one, make multiple measurements on each cell, and then separate the cells viably for further study make this technology unique and indispensable. Our laboratory has long been involved in pushing this technology forward;we have perhaps the most sophisticated flow cytometer in the world, currently capable of making 20 different measurements on each cell. This platform has proven invaluable in dissecting immune responses to vaccines and viruses. In addition, we are developing novel algorithms for the automated analysis of the highly complex data that results from these experiments.

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Finak, Greg; McDavid, Andrew; Chattopadhyay, Pratip et al. (2014) Mixture models for single-cell assays with applications to vaccine studies. Biostatistics 15:87-101
Mahnke, Yolanda D; Beddall, Margaret H; Roederer, Mario (2013) OMIP-015: human regulatory and activated T-cells without intracellular staining. Cytometry A 83:179-81
Dominguez, Maria H; Chattopadhyay, Pratip K; Ma, Steven et al. (2013) Highly multiplexed quantitation of gene expression on single cells. J Immunol Methods 391:133-45
Mahnke, Yolanda D; Beddall, Margaret H; Roederer, Mario (2013) OMIP-017: human CD4(+) helper T-cell subsets including follicular helper cells. Cytometry A 83:439-40
Mahnke, Yolanda D; Beddall, Margaret H; Roederer, Mario (2013) OMIP-019: quantification of human ??T-cells, iNKT-cells, and hematopoietic precursors. Cytometry A 83:676-8
Nguyen, Richard; Perfetto, Stephen; Mahnke, Yolanda D et al. (2013) Quantifying spillover spreading for comparing instrument performance and aiding in multicolor panel design. Cytometry A 83:306-15
Zarkowsky, David; Lamoreaux, Laurie; Chattopadhyay, Pratip et al. (2011) Heavy metal contaminants can eliminate quantum dot fluorescence. Cytometry A 79:84-9
Roederer, Mario; Nozzi, Joshua L; Nason, Martha C (2011) SPICE: exploration and analysis of post-cytometric complex multivariate datasets. Cytometry A 79:167-74
Gratama, Jan W; Kern, Florian; Manca, Fabrizio et al. (2008) Measuring antigen-specific immune responses, 2008 update. Cytometry A 73:971-4
Chattopadhyay, Pratip K; Melenhorst, J Joseph; Ladell, Kristin et al. (2008) Techniques to improve the direct ex vivo detection of low frequency antigen-specific CD8+ T cells with peptide-major histocompatibility complex class I tetramers. Cytometry A 73:1001-9

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