Eighty to ninety percent of what most young children learn about the world comes through vision. The same cannot be said when we seek to learn about the inner workings of our own body, because light beyond """"""""""""""""skin deep"""""""""""""""" becomes diffused due to multiple scattering. Instead, researchers have resorted to alternative means-such as X-ray, magnetic resonance, and ultrasound-to probe deep into the body. Until now, most advances in optical imaging have been geared towards high-resolution functional and molecular imaging at depths less than 1 mm in scattering tissue. The pursuit of deep-tissue optical imaging with high spatial resolution has been stymied by the inherent optical diffusion-the grand challenge since the inception of biomedical optics. We must meet this challenge to reach the full potential of light because it is such a powerful tool from both the physical and biological perspectives. Physically, the tiny fraction of the electromagnetic spectrum that light covers is the only part that probes molecular structures directly;biologically, the ability of molecules to sense, react to, and emit light is encoded on the most fundamental (i.e., genetic) level! In addition, light as nonionizing radiation is as safe to biological organisms as air and water. Therefore, light is the most natural choice fo visualizing biological structures and events, interrogating and controlling biological processes, as well as diagnosing and treating diseases, if only we could overcome the optical diffusion-a seemingly unbreakable barrier. While multiple scattering of light is treated as a problem in conventional wisdom, I believe that it should be part of the solution. Our recent work on time-reversed ultrasonically encoded (TRUE) optical focusing (Nature Photonics 2011) is a first breakthrough in this direction. TRUE focusing can noninvasively deliver light to a dynamically defined focus deep in a scattering medium. This invention opens the door to an even greater paradigm-shifti

National Institute of Health (NIH)
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
NIH Director’s Pioneer Award (NDPA) (DP1)
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Special Emphasis Panel (ZGM1-NDPA-A (01))
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Conroy, Richard
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Washington University
Biomedical Engineering
Schools of Engineering
Saint Louis
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Hsu, Hsun-Chia; Li, Lei; Yao, Junjie et al. (2018) Dual-axis illumination for virtually augmenting the detection view of optical-resolution photoacoustic microscopy. J Biomed Opt 23:1-7
Li, Lei; Shemetov, Anton A; Baloban, Mikhail et al. (2018) Small near-infrared photochromic protein for photoacoustic multi-contrast imaging and detection of protein interactions in vivo. Nat Commun 9:2734
Yao, Junjie; Wang, Lihong V (2018) Recent progress in photoacoustic molecular imaging. Curr Opin Chem Biol 45:104-112
Liu, Xiaowei; Wong, Terence T W; Shi, Junhui et al. (2018) Label-free cell nuclear imaging by Grüneisen relaxation photoacoustic microscopy. Opt Lett 43:947-950
Hemphill, Ashton S; Shen, Yuecheng; Hwang, Jeeseong et al. (2018) High-speed alignment optimization of digital optical phase conjugation systems based on autocovariance analysis in conjunction with orthonormal rectangular polynomials. J Biomed Opt 24:1-11
Yang, Jiamiao; Gong, Lei; Shen, Yuecheng et al. (2018) Synthetic Bessel light needle for extended depth-of-field microscopy. Appl Phys Lett 113:181104
Zhang, Pengfei; Li, Lei; Lin, Li et al. (2018) High-resolution deep functional imaging of the whole mouse brain by photoacoustic computed tomography in vivo. J Biophotonics 11:
Liu, Yan; Shen, Yuecheng; Ruan, Haowen et al. (2018) Time-reversed ultrasonically encoded optical focusing through highly scattering ex vivo human cataractous lenses. J Biomed Opt 23:1-4
Cai, De; Wong, Terence T W; Zhu, Liren et al. (2018) Dual-view photoacoustic microscopy for quantitative cell nuclear imaging. Opt Lett 43:4875-4878
Lin, Li; Hu, Peng; Shi, Junhui et al. (2018) Single-breath-hold photoacoustic computed tomography of the breast. Nat Commun 9:2352

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