During the past funding period, we have discovered a number of details of the mechanisms underlying acute ethanol inhibition of voltage-gated calcium channels (VGCC)in PC12 cells, including the involvement of identified G-proteins. We have also discovered that differentiation with nerve growth factor (NGF), as well as chronic exposure to ethanol, significantly decreases acute ethanol inhibition. We plan to focus the next phase of our research on the mechanisms underlying the inhibition of Ga channels. This work, which will use a combination of molecular biology and biophysics, will be performed on three types of preparation, each contributing particular advantages: 1) intact PG12 cells, where we will further elucidate the mechanisms of ethanol action on VGCC; 2) the intact oocyte expression system, where we can study selected populations of channels and components, such as G-proteins, in a cellular environment; and 3)internally perfused oocytes, in which we can manipulate both the interior and exterior environment, maintaining a natural membrane and underlying cytoskeleton. At each level, we will study channels from undifferentiated cells, differentiated cells, and cells chronically exposed to alcohol, allowing us to gain an understanding for the basis of differences in acute sensitivity.

Agency
National Institute of Health (NIH)
Institute
National Institute on Alcohol Abuse and Alcoholism (NIAAA)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
3F31AA005545-01S1
Application #
6130294
Study Section
Special Emphasis Panel (ZAA1 (01))
Program Officer
Yahr, Harold
Project Start
1999-11-30
Project End
Budget Start
1999-08-01
Budget End
2000-07-31
Support Year
1
Fiscal Year
1999
Total Cost
Indirect Cost
Name
University of North Carolina Chapel Hill
Department
Miscellaneous
Type
Schools of Public Health
DUNS #
078861598
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599
Siebert, Darcy Clay; Siebert, Carl F (2007) Help seeking among helping professionals: a role identity perspective. Am J Orthopsychiatry 77:49-55
Siebert, Darcy Clay (2005) Help seeking for AOD misuse among social workers: patterns, barriers, and implications. Soc Work 50:65-75
Siebert, Darcy Clay (2003) Denial of AOD use: an issue for social workers and the profession. Health Soc Work 28:89-97
Cherpitel, C J (1992) Drinking patterns and problems: a comparison of ER patients in an HMO and in the general population. Alcohol Clin Exp Res 16:1104-9
Cherpitel, C J (1988) Drinking patterns and problems associated with injury status in emergency room admissions. Alcohol Clin Exp Res 12:105-10
Stephens, C J (1987) Alcohol consumption and casualties;drinking in the event. Drug Alcohol Depend 20:115-27