Abstract

Neuroblastomas (NB) and Ewing's Sarcoma/peripheral Primitive Neuro-ectodermal Tumors (ES/PNET) are pediatric cancers of neural crest orgin. It has been suggested that the control of the differentiation program o f the sympathetic lineage of NBs versus the parasympathetic lineage of ES/PNETs proceed in a linear fashion. Somatic cell hybridization of NB cell lines and ES/PNET cell lines show that NB-specific markers are repressed, and the transfection of NB with the Ews/FLI-1 chimeric gene characteriztically found in ES/PNETs show similar results. This project will characterize the Ews/FLI-1's role in cell cycle control utilizing western blot analysis to monitor expression of p53 and pRb, immunofluorescence to monitor p53 localization and protein half life experiments to monitor p53 stability. This project will also utilize microchip array techinology to study the mechanism and pathway of the Ews/FLI-1 oncogene.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
5F31CA096576-02
Application #
6647177
Study Section
Special Emphasis Panel (ZRG1-F05 (29))
Program Officer
Bini, Alessandra M
Project Start
2002-09-01
Project End
2004-03-31
Budget Start
2003-09-01
Budget End
2004-03-31
Support Year
2
Fiscal Year
2003
Total Cost
$16,826
Indirect Cost

  Institution

Name
University of North Carolina Chapel Hill
Department
Pharmacology
Type
Schools of Medicine
DUNS #
608195277
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599