Rab 3D- and VAMP2-enriched vesicles represent two potentially distinct populations of secretory vesicles in primary rabbit lacrimal acini. Immunoreactivity associated with these secretory vesicle associated proteins, rab 3D and VAMP2, showed different subcellular distributions when examined under confocal microscopy in both resting and carbachol (100 mM) stimulated cells. Furthermore, isolation of subcellular membrane compartments from CCH-stimulated lacrimal acinar cells by centrifugation over sorbitol density gradients and partitioning into aqueous polyethyleneglycol-dextran followed by SDS-PAGE and Western blotting revealed that much of the rab 3D and VAMP2 immunoreactivity was enriched on distinct membranes. To further test whether these populations are distinct, I propose two specific aims: 1) To determine whether VAMP2- and rab3D-enriched vesicles represent discrete vesicle populations rather than different maturation intermediates by comparing secretagogue sensitivity and the spectrum of secretory products released and 2) To determine whether VAMP2- and rab3D-enriched vesicles represent discrete vesicle populations rather than different maturation intermediates by selectively inhibiting different biosynthetic trafficking processes and evaluating the consequences to each vesicle population. ? ?

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
5F31EY015928-02
Application #
6919860
Study Section
Special Emphasis Panel (ZRG1-CDF-1 (29))
Program Officer
Fisher, Richard S
Project Start
2004-07-01
Project End
2007-06-30
Budget Start
2005-07-01
Budget End
2006-06-30
Support Year
2
Fiscal Year
2005
Total Cost
$28,055
Indirect Cost
Name
University of Southern California
Department
Pharmacology
Type
Schools of Pharmacy
DUNS #
072933393
City
Los Angeles
State
CA
Country
United States
Zip Code
90089
Kadoch, V; Bodin, F; Himy, S et al. (2010) Latissimus dorsi free flap for reconstruction of extensive full-thickness abdominal wall defect. A case of desmoid tumor. J Visc Surg 147:e45-8
Marchelletta, Ronald R; Jacobs, Damon T; Schechter, Joel E et al. (2008) The class V myosin motor, myosin 5c, localizes to mature secretory vesicles and facilitates exocytosis in lacrimal acini. Am J Physiol Cell Physiol 295:C13-28