A growing number of diseases, including neuropathology and developmental disorders, are thought to result from disrupted transport of organelles. Melanosome transport in pigment cells is an ideal model system for studying the molecular mechanisms of organelle transport. We propose to examine the regulation of molecular motor proteins by applying genetics to the problem of melanosome transport in zebrafish melanophores. This will be accomplished by participating in two on going mutagenesis screens and screening zebrafish mutants specifically for alterations in melanosome dynamics. Mutants of interest will then be further examined by isolating their melanophores and, via live cell imaging, characterizing their defects in pigment transport through an assay system we have developed. Once we have identified mutants that are defective in microtubule pigment transport we will clone the genes responsible and examine their role in regulating motor activity.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Predoctoral Individual National Research Service Award (F31)
Project #
5F31GM071198-03
Application #
7115915
Study Section
Special Emphasis Panel (ZRG1-F05 (20))
Program Officer
Gaillard, Shawn R
Project Start
2004-09-01
Project End
2007-01-14
Budget Start
2006-09-01
Budget End
2007-01-14
Support Year
3
Fiscal Year
2006
Total Cost
$18,142
Indirect Cost
Name
Oregon Health and Science University
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
096997515
City
Portland
State
OR
Country
United States
Zip Code
97239
Sheets, Lavinia; Ransom, David G; Mellgren, Eve M et al. (2007) Zebrafish melanophilin facilitates melanosome dispersion by regulating dynein. Curr Biol 17:1721-34