It is the overall goal of this proposed research to better understand the role of Iroquois (Irx) transcription factors in the differentiation of the atrium and ventricle during cardiac development. Specifically, by misexpressing Irx4 throughout the heart in mice, we will determine whether a ventricular program of gene expression can be imposed in the atrium. Conversely, with a combination of multiple Irx-gene knockouts and the expression of a dominant negative Irx4 molecule in the ventricle, we will determine whether genetic redundancy with other Irx family members exists in the heart. Finally, we will identify enhancer elements that control ventricle-specific Irx4 expression. This combination of experimental approaches will reveal important insight into the regulation of ventricular versus atrial chamber identity, and increase the understanding of the molecular basis of congenital heart defects and abnormal heart function.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
1F32HL077047-01
Application #
6791005
Study Section
Special Emphasis Panel (ZRG1-F05 (20))
Program Officer
Commarato, Michael
Project Start
2004-05-07
Project End
2005-03-14
Budget Start
2004-05-07
Budget End
2005-03-14
Support Year
1
Fiscal Year
2004
Total Cost
$30,628
Indirect Cost
Name
Hospital for Sick Chldrn (Toronto)
Department
Type
DUNS #
208511808
City
Toronto
State
ON
Country
Canada
Zip Code
M5 1-X8