It is the overall goal of this proposed research to better understand the role of Iroquois (Irx) transcription factors in the differentiation of the atrium and ventricle during cardiac development. Specifically, by misexpressing Irx4 throughout the heart in mice, we will determine whether a ventricular program of gene expression can be imposed in the atrium. Conversely, with a combination of multiple Irx-gene knockouts and the expression of a dominant negative Irx4 molecule in the ventricle, we will determine whether genetic redundancy with other Irx family members exists in the heart. Finally, we will identify enhancer elements that control ventricle-specific Irx4 expression. This combination of experimental approaches will reveal important insight into the regulation of ventricular versus atrial chamber identity, and increase the understanding of the molecular basis of congenital heart defects and abnormal heart function.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Postdoctoral Individual National Research Service Award (F32)
Project #
5F32HL077047-03
Application #
6899709
Study Section
Special Emphasis Panel (ZRG1-F05 (20))
Program Officer
Meadows, Tawanna
Project Start
2004-05-07
Project End
2007-05-06
Budget Start
2005-05-07
Budget End
2006-05-06
Support Year
3
Fiscal Year
2005
Total Cost
$43,976
Indirect Cost
Name
University of Texas Sw Medical Center Dallas
Department
Biochemistry
Type
Schools of Medicine
DUNS #
800771545
City
Dallas
State
TX
Country
United States
Zip Code
75390