This application is concerned with advancing the scientific experience and development of the candidate while developing a comprehensive understanding of the cryobiology of felid sperm. The ultimate research aim is to improve the efficiency of assisted reproduction, specifically artificial insemination (AI), for propagating cats valuable to biomedical research and for conserving endangered felids. Domesticated cat models for human disease and endangered felid species frequently experience poor reproductive success, physical or behavioral incompatibilities, and abnormal sperm traits. AI with cryopreserved sperm could circumvent these problems. However, lack of knowledge about the cryobiology for felid sperm has hindered effective use of these powerful tools. This application describes basic and applied studies involving domestic cat populations that experience normospermia (>60% morphologically normal sperm) or teratospermia (<40% normal sperm), a condition commonly observed in rare felids. Findings also will be compared to wild felids that are normospermic (leopard cat), or teratospermic (clouded leopard) nondomestic felids. Comparative studies will focus on the impact of cryoprotectant and temperature on cat sperm physiology and function, specifically: 1) physical and biomedical properties of sperm plasma membranes pertaining to cryodamage sensitivity; 2) stabilization of sperm function; and 3) identification of cryopreservation conditions that maximize sperm function. Studies will identify variables most influential to post-thaw sperm survival, allowing an optimal laboratory protocol to be developed and tested across cat populations and species. The best method will be used systematically for AI in partnership with institutions holding the rarest cats of value to biomedical research and a host of selected endangered species. This training/research approach will allow the candidate career advancement as a reproductive specialist in laboratory and rare felid species. Extensive interactions with eight investigators at seven institutions will provide access to fundamental research concepts of cell biology and sophisticated techniques, including membrane lipidology, cell volume measurements, flow cytometry, cryomicroscopy and AI. Finally, the research will determine the factors influencing cryosurvival of felid sperm while identifying sperm cryobank protocols that will lead to more efficient and effective propagation and conservation of rare felids.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Research Scientist Development Award - Research &amp; Training (K01)
Project #
5K01RR000135-05
Application #
6540543
Study Section
National Center for Research Resources Initial Review Group (RIRG)
Program Officer
Watson, William T
Project Start
1998-05-01
Project End
2005-04-30
Budget Start
2002-05-01
Budget End
2005-04-30
Support Year
5
Fiscal Year
2002
Total Cost
$116,429
Indirect Cost
Name
Smithsonian Institution
Department
Type
DUNS #
City
Arlington
State
VA
Country
United States
Zip Code
22202
Shah, Arya; Hoffman, E Matthew; Mauermann, Michelle L et al. (2018) Incidence and disease burden of chemotherapy-induced peripheral neuropathy in a population-based cohort. J Neurol Neurosurg Psychiatry 89:636-641
Eirin, Alfonso; Zhu, Xiang-Yang; Ferguson, Christopher M et al. (2015) Intra-renal delivery of mesenchymal stem cells attenuates myocardial injury after reversal of hypertension in porcine renovascular disease. Stem Cell Res Ther 6:7
Pelican, Katharine M; Spindler, Rebecca E; Pukazhenthi, Budhan S et al. (2010) Progestin exposure before gonadotropin stimulation improves embryo development after in vitro fertilization in the domestic cat. Biol Reprod 83:558-67
Crosier, Adrienne E; Marker, Laurie; Howard, JoGayle et al. (2007) Ejaculate traits in the Namibian cheetah (Acinonyx jubatus): influence of age, season and captivity. Reprod Fertil Dev 19:370-82
Crosier, Adrienne E; Pukazhenthi, Budhan S; Henghali, Josephine N et al. (2006) Cryopreservation of spermatozoa from wild-born Namibian cheetahs (Acinonyx jubatus) and influence of glycerol on cryosurvival. Cryobiology 52:169-81
Pukazhenthi, Budhan S; Neubauer, Katrin; Jewgenow, Katarina et al. (2006) The impact and potential etiology of teratospermia in the domestic cat and its wild relatives. Theriogenology 66:112-21
Pukazhenthi, Budhan; Laroe, Debra; Crosier, Adrienne et al. (2006) Challenges in cryopreservation of clouded leopard (Neofelis nebulosa) spermatozoa. Theriogenology 66:1790-6
Comizzoli, Pierre; Wildt, David E; Pukazhenthi, Budhan S (2004) Effect of 1,2-propanediol versus 1,2-ethanediol on subsequent oocyte maturation, spindle integrity, fertilization, and embryo development in vitro in the domestic cat. Biol Reprod 71:598-604
Neubauer, Katrin; Jewgenow, Katarina; Blottner, Steffen et al. (2004) Quantity rather than quality in teratospermic males: a histomorphometric and flow cytometric evaluation of spermatogenesis in the domestic cat (Felis catus). Biol Reprod 71:1517-24
Comizzoli, P; Wildt, D E; Pukazhenthi, B S (2003) Overcoming poor in vitro nuclear maturation and developmental competence of domestic cat oocytes during the non-breeding season. Reproduction 126:809-16

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