During the tenure of a K04, I intend to pursue the following specific questions: 1. Analyze the molecular biology of normal, mutant, and retinoic-acid-induced cell death in the interdigital region of the developing mouse by examination of light- and electron microscopic morphology, DNA fragmentation; RNA and protein synthesis, and expression of genes associated with cell death such as transglutaminase, TRPM-2 and Bcl-2). 2) Establish an in vitro organ culture system as a model for studying limb development and cell death. II. Isolate genes related to programmed cell death: A. Establishment of stage-specific cDNA libraries for isolation of developmental stage specific and cell death related genes. B. Isolate and characterize common cDNAs activated during involution of Manduca labial gland and muscle.

Agency
National Institute of Health (NIH)
Institute
National Institute on Aging (NIA)
Type
Modified Research Career Development Award (K04)
Project #
5K04AG000631-02
Application #
2048450
Study Section
Molecular Cytology Study Section (CTY)
Project Start
1994-05-01
Project End
1999-04-30
Budget Start
1995-05-01
Budget End
1996-04-30
Support Year
2
Fiscal Year
1995
Total Cost
Indirect Cost
Name
Queens College
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
City
Flushing
State
NY
Country
United States
Zip Code
11367
Lockshin, R A; Facey, C O; Zakeri, Z (2001) Cell death in the heart. Cardiol Clin 19:1-11
Kagan, T; Davis, C; Lin, L et al. (1999) Coenzyme Q10 can in some circumstances block apoptosis, and this effect is mediated through mitochondria. Ann N Y Acad Sci 887:31-47
Ahuja, H S; Zhu, Y; Zakeri, Z (1997) Association of cyclin-dependent kinase 5 and its activator p35 with apoptotic cell death. Dev Genet 21:258-67
Ahuja, H S; James, W; Zakeri, Z (1997) Rescue of the limb deformity in hammertoe mutant mice by retinoic acid-induced cell death. Dev Dyn 208:466-81
Ahuja, H S; Tenniswood, M; Lockshin, R et al. (1994) Expression of clusterin in cell differentiation and cell death. Biochem Cell Biol 72:523-30