During the tenure of a K04, I intend to pursue the following specific questions: 1. Analyze the molecular biology of normal, mutant, and retinoic-acid-induced cell death in the interdigital region of the developing mouse by examination of light- and electron microscopic morphology, DNA fragmentation; RNA and protein synthesis, and expression of genes associated with cell death such as transglutaminase, TRPM-2 and Bcl-2). 2) Establish an in vitro organ culture system as a model for studying limb development and cell death. II. Isolate genes related to programmed cell death: A. Establishment of stage-specific cDNA libraries for isolation of developmental stage specific and cell death related genes. B. Isolate and characterize common cDNAs activated during involution of Manduca labial gland and muscle.
| Lockshin, R A; Facey, C O; Zakeri, Z (2001) Cell death in the heart. Cardiol Clin 19:1-11 |
| Kagan, T; Davis, C; Lin, L et al. (1999) Coenzyme Q10 can in some circumstances block apoptosis, and this effect is mediated through mitochondria. Ann N Y Acad Sci 887:31-47 |
| Ahuja, H S; Zhu, Y; Zakeri, Z (1997) Association of cyclin-dependent kinase 5 and its activator p35 with apoptotic cell death. Dev Genet 21:258-67 |
| Ahuja, H S; James, W; Zakeri, Z (1997) Rescue of the limb deformity in hammertoe mutant mice by retinoic acid-induced cell death. Dev Dyn 208:466-81 |
| Ahuja, H S; Tenniswood, M; Lockshin, R et al. (1994) Expression of clusterin in cell differentiation and cell death. Biochem Cell Biol 72:523-30 |
