The long-term goal of the research in my laboratory is to gain a molecular understanding of the role glial cells (specifically Schwann cells and oligodendrocytes) play in the development and function of the nervous system. Our principal approach is to use techniques that allow for the manipulation of the mouse genome. This approach has allowed us to generate a mouse model of human oligodendrogliomas - which in turn has been useful for the establishment of an oligodendroglial cell line. Moreover, we have developed a transgenic line of animals that experience a severe, late-onset neuromuscular disorder that is likely the result of abnormal Schwann - cell axon interactions. In addition to our transgenic experiments, we have recently initiated studies using the polymerase chain reaction in an effort to isolate genes encoding members of protein families that likely play a role in glial cell function.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Modified Research Career Development Award (K04)
Project #
1K04NS001637-01
Application #
3075224
Study Section
Neurology C Study Section (NEUC)
Project Start
1993-04-01
Project End
1998-03-31
Budget Start
1993-04-01
Budget End
1994-03-31
Support Year
1
Fiscal Year
1993
Total Cost
Indirect Cost
Name
University of North Carolina Chapel Hill
Department
Type
Schools of Medicine
DUNS #
078861598
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599
Baerwald, K D; Popko, B (1998) Developing and mature oligodendrocytes respond differently to the immune cytokine interferon-gamma. J Neurosci Res 52:230-9
Dupree, J L; Suzuki, K; Popko, B (1998) Galactolipids in the formation and function of the myelin sheath. Microsc Res Tech 41:431-40
Coetzee, T; Suzuki, K; Popko, B (1998) New perspectives on the function of myelin galactolipids. Trends Neurosci 21:126-30
Taniike, M; Marcus, J R; Popko, B et al. (1997) Expression of major histocompatibility complex class I antigens in the demyelinating twitcher CNS and PNS. J Neurosci Res 47:539-46
Popko, B; Corbin, J G; Baerwald, K D et al. (1997) The effects of interferon-gamma on the central nervous system. Mol Neurobiol 14:19-35
Coetzee, T; Li, X; Fujita, N et al. (1996) Molecular cloning, chromosomal mapping, and characterization of the mouse UDP-galactose:ceramide galactosyltransferase gene. Genomics 35:215-22
Corbin, J G; Kelly, D; Rath, E M et al. (1996) Targeted CNS expression of interferon-gamma in transgenic mice leads to hypomyelination, reactive gliosis, and abnormal cerebellar development. Mol Cell Neurosci 7:354-70
Goodrum, J F; Bouldin, T W; Zhang, S H et al. (1995) Nerve regeneration and cholesterol reutilization occur in the absence of apolipoproteins E and A-I in mice. J Neurochem 64:408-16
Li, Y; Atashi, J; Hayes, C et al. (1995) Morphological and molecular response of the MOCH-1 oligodendrocyte cell line to serum and interferon-gamma: possible implications for demyelinating disorders. J Neurosci Res 40:189-98
Rath, E M; Kelly, D; Bouldin, T W et al. (1995) Impaired peripheral nerve regeneration in a mutant strain of mice (Enr) with a Schwann cell defect. J Neurosci 15:7226-37

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