The overall aim of this project is the functional characterization of cell surface antigens expressed on activated but not resting human B lymphocytes. These antigens are excellent candidates for cell surface structures which control B cell activation, proliferation, and differentiation. This proposal will involve a detailed analysis of B cell activation antigens with specific attention to: 1) the stimuli which induce their expression, 2) their relationship to other B cell antigens expressed on both resting and activated B cells, 3) their relationship to growth and differentiation factors which are involved in the proliferation of B cells and stimulation of immunoglobulin secretion, and 4) the expression of these antigens on neoplastic B cells, and their possible role in neoplastic B cell proliferation. To accomplish this, monoclonal antibodies to previously known and new B cell activation antigens will be developed, and extensively characterized. The expression of these antigens will be related to the stages of B cell activation/proliferation and differentiation, and more importantly an attempt will be made to define the functional importance of these antigens. Finally, the expression of B cell activation antigens will be examined on B cell leukemias and lymphomas. This will be done in order to determine whether there is a correlation between antigen expression on neoplastic B cells and both the ability to respond to physiologic triggers of activation/proliferation in vitro as well as the clinical presentation and course of disease.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Clinical Investigator Award (CIA) (K08)
Project #
1K08CA001105-01
Application #
3079604
Study Section
(SRC)
Project Start
1986-08-01
Project End
1989-07-31
Budget Start
1986-08-01
Budget End
1987-07-31
Support Year
1
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Danbury Hospital
Department
Type
DUNS #
City
Danbury
State
CT
Country
United States
Zip Code
06810
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