Abstract

Research: This research proposal focuses on the role of novel protein kinases in human breast carcinoma. Using PCR technology, cDNA libraries of related kinases will be generated from breast cancer specimens. In a preliminary library from a breast cancer cell line, several novel tyrosine and serine/threonine kinase genes have been isolated. As the novel kinases are discovered, their role in breast carcinoma will be studied by examining expression in breast tumor samples and producing antibodies to recombinant novel kinase peptides. This will allow assessment of both the levels of expression and the specific activity of the kinase proteins in human breast carcinoma. Isolation of the full-length cDNA clones will be performed on those kinases which appear to play a role in breast carcinogenesis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Clinical Investigator Award (CIA) (K08)
Project #
5K08CA001625-02
Application #
3080085
Study Section
Cancer Institutional Fellowship Review Committee (CT)
Project Start
1992-08-01
Project End
1997-07-31
Budget Start
1993-08-01
Budget End
1994-07-31
Support Year
2
Fiscal Year
1993
Total Cost
Indirect Cost

  Institution

Name
University of North Carolina Chapel Hill
Department
Type
Schools of Medicine
DUNS #
078861598
City
Chapel Hill
State
NC
Country
United States
Zip Code
27599

  Publications

Xu, L H; Owens, L V; Sturge, G C et al. (1996) Attenuation of the expression of the focal adhesion kinase induces apoptosis in tumor cells. Cell Growth Differ 7:413-8
Owens, L V; Xu, L; Dent, G A et al. (1996) Focal adhesion kinase as a marker of invasive potential in differentiated human thyroid cancer. Ann Surg Oncol 3:100-5
Owens, L V; Xu, L; Craven, R J et al. (1995) Overexpression of the focal adhesion kinase (p125FAK) in invasive human tumors. Cancer Res 55:2752-5
Craven, R J; Cance, W G; Liu, E T (1995) The nuclear tyrosine kinase Rak associates with the retinoblastoma protein pRb. Cancer Res 55:3969-72
Craven, R J; Xu, L H; Weiner, T M et al. (1995) Receptor tyrosine kinases expressed in metastatic colon cancer. Int J Cancer 60:791-7
Cance, W G; Craven, R J; Bergman, M et al. (1994) Rak, a novel nuclear tyrosine kinase expressed in epithelial cells. Cell Growth Differ 5:1347-55
Levedakou, E N; He, M; Baptist, E W et al. (1994) Two novel human serine/threonine kinases with homologies to the cell cycle regulating Xenopus MO15, and NIMA kinases: cloning and characterization of their expression pattern. Oncogene 9:1977-88
Weiner, T M; Liu, E T; Craven, R J et al. (1994) Expression of growth factor receptors, the focal adhesion kinase, and other tyrosine kinases in human soft tissue tumors. Ann Surg Oncol 1:18-27
Cance, W G; Craven, R J; Weiner, T M et al. (1993) Novel protein kinases expressed in human breast cancer. Int J Cancer 54:571-7