The proposed research is directed at enhancing the understanding of factors regulating the attachment of gingival fibroblasts (GF) and periodontal ligament cells (PDL cells) to a substratum, in vitro. The attachment of periodontal cells at the healing site is one of the initial requirements for successful tissue regeneration.
The specific aims are to determine the biochemical mechanisms regulating the interaction of bone sialoprotein I (BSP I), a known attachment protein, with GF and PDL cell surfaces by: 1) determining the mechanism(s) regulating BSP I persistent attachment effect upon GF.2) determining whether BSP I must bind to bone specific proteoglycans in order to promote cell attachment. 3) identifying the cell receptor(s) for BSP I on GF and PDL cells. 4) determining cell surface distribution of BSP I receptors by preparing antibodies to the BSP I receptors. The studies proposed here will provide important information necessary to achieve the ultimate clinical goal of periodontal regeneration. Moreover, these studies will provide new knowledge on the cellular and molecular biology of human tissue regeneration.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Unknown (K15)
Project #
1K15DE000262-01
Application #
3088606
Study Section
NIDR Special Grants Review Committee (DSR)
Project Start
1989-07-01
Project End
1994-06-30
Budget Start
1989-07-01
Budget End
1990-06-30
Support Year
1
Fiscal Year
1989
Total Cost
Indirect Cost
Name
University of Maryland Baltimore
Department
Type
Schools of Dentistry/Oral Hygn
DUNS #
003255213
City
Baltimore
State
MD
Country
United States
Zip Code
21201
Somerman, M J; Shroff, B; Agraves, W S et al. (1990) Expression of attachment proteins during cementogenesis. J Biol Buccale 18:207-14